针刺对慢性情绪应激焦虑大鼠血浆ANP、CORT和肾上腺ANP受体的影响  

Effects of Acupuncture on ANP and CORT in Plasma for Chronic Emotional Stress Anxious Rats

作　　者：魏大能[1] 张承舜[1] 吕鹏[1] 赵纪岚[1] 蔡定均[1] 余曙光[1] 周奇志[1] 张潇丹[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《辽宁中医杂志》2017年第5期1074-1077,I0002,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173322);教育部博士点基金专项科研基金博导类课题(20135132110009)

摘　　要：目的:通过观察针刺对慢性情绪应激焦虑大鼠血浆心房利钠肽(atrial natriuretic peptide,ANP)、皮质酮(corticosterone,CORT)含量和肾上腺利钠肽A型受体(natriuretic peptide receptor type A,NPR-A)水平的影响,探讨针刺干预焦虑障碍模型的部分外周作用机理。方法:选取清洁级雄性SD大鼠30只,完全随机分为空白组、模型组、针刺组3组。运用不可预知的慢性情绪应激法建立焦虑动物模型,针刺组同时选取"内关""神门"予以治疗。以高架十字迷宫实验(elevated plus maze test,EPM)测试大鼠焦虑样行为,分别采用放射免疫法和ELISA法检测血浆ANP和CORT的含量,运用免疫组化法检测肾上腺皮质部NPR-A的水平。结果:与空白组对比,模型组的进入开臂次数(open-arms entries,OE)占入臂总次数的百分比值(OE%)显著下降(P<0.01),血浆ANP、CORT值均明显升高(P<0.05或P<0.01);与模型组相比,针刺组OE%值有升高并接近空白组,血浆CORT显著下降(P<0.01);3组肾上腺皮质NPR-A水平无显著性差异。结论:针刺可能通过降低血浆ANP和CORT的含量达到改善焦虑情绪的目的,而肾上腺皮质ANP受体的参与作用未显现。Objective ：To observe the effect of acupuncture on the contents of atrial natriuretic peptide （ANP） ,corticosterone （CORT） in plasma and the level of natriuretic peptide receptor type A （NPR -A） in adrenal gland with chronic emotional stress （CES） anxious rats and explore the partial peripheral mechanism of anxiolytic effect of acupuncture. Methods ： Thirty healthy male SD rats were randomly divided into control, model and acupuncture groups, and unpredictable chronic emotional stress was con- ducted to establish CES anxious model rats, meanwhile ＂Neiguan＂ （PC6） and ＂Shenmen＂ （ HT7 ） acupoints were stimulated in acupuncture group. Elevated plus maze （EPM） was utilized to test the behavioral changes of rats. The levels of ANP in plasma were detected by radioimmunoassay and CORT by ELISA and the level of NPR - A in adrenal cortex was examined by immunohis- tochemistry. Results ： The OE% value of the model group was significantly lower than that of the control group （ P 〈 0. 05 ）, but the concentrations of ANP and CORT in plasma were higher than those of the control group （P 〈0. 05 or P 〈 0. 01 ）. Compared with model group, the content of plasma CORT in the acupuncture group was obviously decreased （ P 〈 0. 05 ） but ANP was reduced a little （ P 〉 0. 05 ）. And the NPR - A of adrenal cortex was not expressed obviously among those three groups. Conclusion ： These resuits suggested that acupuncture had anxiolytic effects through reducing the ANP and CORT in plasma, but the ANP receptor in adrenal cortex did not participate in the regulation of anxiety - like behavior.

关 键 词：针刺 焦虑 ANP NPR-A CORT 

分 类 号：R749.72[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]