复方竹叶石膏颗粒对家兔急性放射性食管损伤Bcl-2与Bax mRNA及蛋白表达的影响  

Effects of Compound Zhuye Shigao Granule on mRNA and Protein Expressions of Bcl-2 and Bax in Treatment of Rabbits with Radiation Esophagitis

作　　者：王丽娟[1] 路军章[1] 李明伟[1] 陈格格 牛保龙[2] 曲宝林[2] 

机构地区：[1]解放军总医院中医科,北京100853 [2]解放军总医院放疗科,北京100853

出　　处：《解放军医药杂志》2017年第5期1-4,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然基金课题资助项目(81373543)

摘　　要：目的探讨复方竹叶石膏颗粒对家兔急性放射性食管炎组织中Bax和Bcl-2 mRNA及蛋白表达的影响。方法将40只雄性家兔随机分为空白对照组(A组)、单纯照射组(B组)、复方竹叶石膏颗粒组(C组)、康复新液组(D组),每组10只。B、C、D组均用6 m V-X射线对家兔食管单次照射32 Gy。C、D组于照射前1周照射至放疗结束后7 d。4组均于照射后第7天留取家兔全长食管,检测Bax和Bcl-2 mRNA和蛋白的表达水平。结果 B组家兔食管组织中Bax mRNA与蛋白表达水平较A组升高,Bcl-2 mRNA与蛋白表达及Bcl-2/Bax值较A组降低(P<0.05)。C、D组Bcl-2 mRNA与蛋白表达水平及Bcl-2/Bax值高于B组,Bax mRNA与蛋白表达水平低于B组(P<0.05)。C组Bcl-2 mRNA与蛋白表达水平均高于D组(P<0.05)。结论复方竹叶石膏颗粒能够防治急性放射性食管炎,其机制可能与上调Bcl-2基因与下调Bax基因的表达有关。Objective To investigate effects of Compound Zhuye Shigao Granule （CZYG） on mRNA and protein expressions of bcl-2 and bax in treatment of rabbits with radiation esophagitis. Methods A total of 40 male rabbits were randomly divided into control group （n = 10, group A）, simple radiation group （n = 10, group B）, CZYG group （n = 10, group C） and Kangfuxinye group （n = 10, group D）. All rabbits except those in control group were irradiated by 6MV X-ray at a dose of 32Gy for one time. The drugs were given starting from 1 week before the irradiation to at 7th d after irradiation in group C and D. Samples of the total length of rabbits＇esophagus were collected at 7th d after irradiation in all groups to detect levels of mRNA and protein expressions of bcl-2 and bax. Results In group B, Bax mRNA and protein expressions were significantly increased, while values of Bcl-2 mRNA and protein expressions and Bcl-2/Bax were significantly decreased compared with those in group B （P 〈 0. 05）. Compared with those in group B, values of Bcl-2 mRNA and protein expressions and Bcl-2/Bax were significantly increased , while Bax mRNA and protein expressions were significantly decreased in group C and D （P 〈0. 05）. Bcl-2 mRNA and protein expressions in group C were significantly increased compared with those in group D （P 〈 0.05）. Conclusion Compound Zhuye Shigao Granule （CZYG） can prevent radiation esophagitis, and its mechanism may be related to increase Bcl-2 expression and decrease Bax ex- pression.

关 键 词：食管炎 放射性 复方竹叶石膏颗粒 细胞凋亡 兔 

分 类 号：R-332[医药卫生] R818.84