复方竹叶石膏颗粒对家兔急性放射性食管炎COX-2与MMP-2 mRNA及蛋白表达的影响  

Effects of Compound Zhuye Shigao Granule on mRNA and Protein Expressions of COX-2 and MMP-2 in Treatment of Rabbits with Radiation Esophagitis

作　　者：王丽娟[1] 路军章[1] 李明伟[1] 陈格格 牛保龙[2] 曲宝林[2] 

机构地区：[1]解放军总医院中医科,北京100853 [2]解放军总医院放疗科,北京100853

出　　处：《解放军医药杂志》2017年第5期5-8,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然基金课题资助项目(81373543)

摘　　要：目的探讨复方竹叶石膏颗粒对家兔急性放射性食管炎环氧化酶-2(COX-2)与基质金属蛋白酶-2(MMP-2)mRNA及蛋白表达的影响。方法将40只雄性家兔随机分为空白对照组、单纯照射组、复方竹叶石膏颗粒组、康复新液组,每组10只。空白对照组不做任何处理,其余3组均给予6 m V-X射线对家兔食管单次照射32 Gy。给药组于照射前1周给药至照射结束后7 d,4组均于照射后第7天留取家兔全长食管,检测COX-2与MMP-2 mRNA及蛋白表达情况。结果单纯照射组中COX-2与MMP-2 mRNA与蛋白水平高于空白对照组,复方竹叶石膏颗粒组和康复新液组均低于单纯照射组,且复方竹叶石膏颗粒组低于康复新液组(P<0.05)。结论复方竹叶石膏颗粒能降低COX-2与MMP-2 mRNA与蛋白表达水平,从而发挥对急性放射性食管炎的防治作用。Objective To investigate effects of Compound Zhuye Shigao Granule （CZYG） on mRNA and protein expressions of cycloxygenase-2 （COX-2） and matrix metalloproteinases-2 （MMP-2） in treatment of rabb＇its with radiation esophagitis. Methods A total of 40 male rabbits were randomly divided into control group （n = 10, group A）, simple radiation group （n = 10, group B）, CZYG group （n = 10, group C） and Kangfuxinye group （n = 10, group D）. All rabbits except those in control group were irradiated by 6MV X-ray at a dose of 32Gy for one time. The drugs were given starting from 1 week before the irradiation to at 7thd after irradiation in group C and D. Samples of the total length of rab- bits＇esophagus were collected at 7th d after irradiation in all groups to detect levels of mRNA and protein expressions of COX-2 and MMP-2. Results In group B, mRNA and protein expressions of COX-2 and MMP-2 were significantly in- creased compared with those in group A （P 〈 0. 05）, While the expressions in group C and D were significantly lower than those in group B, and the expressions in group C were significantly lower than those in group D （P 〈0. 05）. Conclusion Compound Zhuye Shigao Granule can prevent radiation esophagitis by decreasing mRNA and protein expressions of COX-2 and MMP-2.

关 键 词：食管炎 放射性 复方竹叶石膏颗粒 环氧化酶-2 基质金属蛋白酶-2 兔 

分 类 号：R-332[医药卫生] R818.71