人肝癌相关成纤维细胞与肝癌血管生成的相关性研究  

Correlation between human hepatocellular carcinoma associated fibroblast and angiogenesis in hepatocellular carcinoma

作　　者：卢逸[1,2] 林楠[1] 陈之巨[3] 许瑞云[1] 

机构地区：[1]中山大学附属第三医院肝胆外科,广州510630 [2]中山大学附属第三医院肝脏病实验室,广州510630 [3]海南省人民医院胃肠外科,海口570311

出　　处：《中华肝脏外科手术学电子杂志》2017年第3期228-232,共5页Chinese Journal of Hepatic Surgery(Electronic Edition)

基　　金：广东省科技计划项目(2012A020602002;2016A020212004);广东省自然科学基金(2014A030313067;2014A030313144);广东省医学科研基金(A2015116)

摘　　要：目的探讨人原发性肝癌(肝癌)组织中肝癌相关成纤维细胞(hCAF)与肝癌血管生成的关系。方法本前瞻性研究对象为2014年1月至2014年5月在中山大学附属第三医院接受肝癌根治性切除术的20例患者。其中男16例,女4例;年龄35~58岁,中位年龄42岁。患者均签署知情同意书,符合医学伦理学规定。收集患者的一般临床资料和病理资料。采用免疫组化法分别检测肝癌组织中hCAF密度和微血管密度(MVD)。观察hCAF和血管内皮细胞在肝癌组织中的分布,分析hCAF密度、MVD与患者临床病理参数的关系,并分析hCAF密度与MVD的相关性。hCAF密度和MVD与临床病理参数关系分析采用单因素方差分析和LSD-t检验或t检验。hCAF密度与MVD关系分析采用Pearson相关分析。结果肝癌组织中hCAF主要围绕癌血窦分布,血管内皮细胞分布于癌血窦及大血管内壁。癌旁组织中hCAF主要分布于肝血窦Disse间隙内。低分化肝癌组织中hCAF密度为(121±14)个/mm^2,明显高于高、中分化肝癌的(63±9)、(82±11)个/mm^2(LSD-t=11.00,6.90;P<0.05)。癌旁组织为肝硬化时,肝癌组织中hCAF密度为(120±14)个/mm^2,明显高于癌旁组织为慢性肝炎时的(78±9)个/mm^2(t=8.00,P<0.05)。肿瘤直径>5 cm的肝癌组织中MVD为(83±9)个/mm^2,明显高于肿瘤直径≤5 cm的肝癌组织的(32±4)个/mm^2(t=16.40,P<0.05)。低分化肝癌组织中MVD为(61±7)个/mm^2,明显高于高、中分化肝癌的(39±4)、(40±4)个/mm^2(LSD-t=8.60,8.20;P<0.05)。癌旁组织为肝硬化时,肝癌组织中MVD为(63±7)个/mm^2,明显高于癌旁组织为慢性肝炎时的(36±4)个/mm^2(t=10.60,P<0.05)。肝癌MVD与肝癌组织中hCAF密度呈正相关(r=0.92,P<0.05)。结论肝癌组织中hCAF高表达,hCAF与肝癌微血管生成相关,可能共同参与肝癌的发生、发展。Objective To investigate the correlation between human hepatocellular carcinoma associated fibroblast （hCAF） and angiogenesis in the human hepatoeellular carcinoma （HCC） tissues. Methods Twenty patients who underwent radical hepatectomy in the Third Affiliated Hospital of Sun Yat-sen University between January 2014 and May 2014 were enrolled in this prospective study. Among them, 16 were males and 4 females, aged 35-58 years old with a median age of 42 years old. The informed consents of all patients were obtained and the local ethical committee approval was received. Clinical and pathological data of the patients were collected. The hCAF density and microvascular density （MVD） in the HCC tissues were detected by immunohistological staining. The distribution of hCAF and vascular endothelial cells in the HCC tissues was observed. The correlation between hCAF density, MVD and clinicopathological parameters of the patients was analyzed by one-way analysis of variance and LSD-t test or t test. The correlation between hCAF density and MVD was analyzed by Pearson correlation analysis. Results The hCAF in the HCC tissues were mainly distributed around the sinusoid of the tumors, and the vascular endothelial cells were distributed in the sinusoid of the tumors and inner walls of great vessels. The hCAF in the paracarcinoma tissues were mainly distributed in the Disse space of liver sinusoid. The hCAF density in the poorly-differentiated HCC tissues was （121±14）/mm^2, significantly higher than （63±9） and （82±11）/mm^2 in the highly- and moderately-differentiated HCC tissues （LSD-t=ll.00, 6.90; P〈0.05）. When the paracarcinoma tissues progressed into liver cirrhosis, the hCAF density in the HCC tissues was （120=t= 14）/mm^2, significantly higher than （78±9）/mm^2 when the paracarcinoma＇tissues progressed into the chronic hepatitis （t=8.00, P〈0.05）. The MVD in the HCC tissues with tumor diameter 〉5 cm was （83±9）/mm^2, significantly higher than （32±4）/mm^2 in the HCC

关 键 词：肝肿瘤 成纤维细胞 新生血管化 病理性 

分 类 号：R735.7[医药卫生—肿瘤]