CENP-A在口腔鳞状细胞癌中的表达及意义  

Expression and Clinical Significance of Centromere Protein A in Oral Squamous Carcinoma Tissues

作　　者：杨磊[1,2,3] 段晓峰[1,2] 

机构地区：[1]贵州医科大学口腔医学院,贵州贵阳550004 [2]贵州医科大学附院,贵州贵阳550004 [3]贵州医科大学

出　　处：《贵州医科大学学报》2017年第5期566-569,共4页Journal of Guizhou Medical University

基　　金：贵州省科技合作计划项目[黔科合LH字(2015)7420号]

摘　　要：目的:探讨着丝粒蛋白A(CENP-A)在口腔鳞状细胞癌(OSCC)组织中的表达及其与OSCC患者临床病理因素之间的关系。方法:收集32例OSCC组织及同期20例口腔正常组织,采用免疫组织化学染色SP法及实时荧光定量PCR(q PCR)检测CENP-A蛋白及mRNA的表达,观察不同临床病理特征的OSCC组织中CENPA基因表达的差异。结果:与口腔正常组织比较,CENP-A蛋白和mRNA在OSCC组织中的表达均明显升高,差异有统计学意义(P<0.05);OSCC组织中的CENP-A的蛋白和mRNA表达随着肿瘤体积增大、淋巴转移而升高(P<0.05);但与患者年龄、性别及肿瘤分化无关(P>0.05)。结论:OSCC组织中CENP-A蛋白和mRNA的表达明显高于正常组织,其表达与肿瘤体积大小、淋巴转移有关。Objective： To explore the expression level of centromere protein A （ CENP-A ） in oral squamous carcinoma tissues （OSCC） and its relationship with various clinical pathological factors of pa- tients with OSCC. Methods： 32 cases of OSCC tissues and 20 cases of normal oral tissues at the same period were collected. The immunohistoehemistry SP method and real-time quantitative PCR was a- dopted to detect the protein and InRNA expressions of CENP-A. The difference of CENP-A gene ex- pression in OSCC tissues with different clinicopathological features was observed. Results： Compared with normal oral tissues, the expression of CENP-A protein and mRNA in OSCC tissue was significantly increased, and the difference was statistically significant （ P 〈 0.05 ）. The expression of CENP-A pro- tein and mRNA in OSCC increased with the increase of tumor volume and lymph node metastasis （P 〈 0.05 ） but was not related to age, sex and tumor differentiation and stage （ P 〉 0.05 ）. Conclusion ： The protein and mRNA expression of CENP-A in OSCC tissues is significantly higher than that in nor- mal tissues, and the expression is related to tumor size and lymph node metastasis.

关 键 词：着丝粒蛋白A 口腔肿瘤 口腔鳞状细胞癌 免疫组织化学 染色法 

分 类 号：R739.8[医药卫生—肿瘤]