长链非编码RNA在小鼠失神经骨骼肌中的变化规律及意义  

Expression and significance of LncRNA in mouse denervated skeletal muscle

作　　者：韦健[1] 寒桦[2] 梁炳生[1] 

机构地区：[1]山西医科大学第二医院骨科,太原030001 [2]山西医科大学第一医院整形科

出　　处：《中华手外科杂志》2017年第3期213-216,共4页Chinese Journal of Hand Surgery

基　　金：山西医科大学博士启动基金项目（03201421）;国家自然科学基金面上项目（81171722）

摘　　要：目的研究失神经骨骼肌萎缩过程中长链非编码RNA（long non—coding RNA，LncRNA）的表达变化及意义。方法取48只Balb／c小鼠，建立右下肢腓肠肌失神经支配模型，采用实时定量PCR（qPCR）检测失神经支配后1、3、5、7、14和28d的腓肠肌中一些LncRNA是否表达及表达变化。结果实验小鼠腓肠肌失神经支配后的不同时段，LncRNA的Meg3、H19、linc—MD1、Malat1、Neat1、Nctc1、SRA均有表达且表达水平在这段时间的连续观察中基本都呈先升高后降低的趋势。结论LncRNA的Meg3、H19、linc—MD1、Malat1、Neat1、Nctc1、SRA在失神经骨骼肌中有表达，表达强度与肌肉萎缩密切相关。Objective To observe the expression of long non-coding RNA during denervation atrophy of the gastrocnemius muscle and explore its clinical significance. Methods Right gastrocnemius muscle denervation model was created in 48 Balb/c mice. The expression levels of long non- coding RNA in the gastrocnemius were detected by Real-time Quantitative PCR at 1, 3, 5, 7, 14 and 28 days. Results At different time points after denervation, expressions of Meg3, H19, linc-MD1, Malatl, Neatl, Nctcl and SRA were detected. The expression levels increased first and then decreased during the 28 days after denervation. Conclusion The expression of lncRNA （Meg3, H19, linc-MD1, Malatl, Neatl, Nctc 1 and SRA） has a close relationship with skeletal muscle denervation atrophy.

关 键 词：肌萎缩 动物 实验 失神经 长链非编码RNA 

分 类 号：R688[医药卫生—骨科学]