丹参酮ⅡA通过下调survivin促进顺铂对咽鳞状细胞癌Fadu细胞抑制作用的研究  

The effect of tanshinone ⅡA potentiates the effects of Cisplatin in Fadu cells in vitro through downregulation of survivin

作　　者：赵艳霞[1] 罗丹[1] 张彦惠[1] 沈斌[1] 王保鑫 孙臻峰[1] 

机构地区：[1]上海交通大学附属第一人民医院耳鼻咽喉-头颈外科,上海200080

出　　处：《临床耳鼻咽喉头颈外科杂志》2017年第10期781-784,788,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：上海市自然科学基金(No:122R1424700)

摘　　要：目的:研究丹参酮ⅡA(TanⅡA)联合顺铂(DDP)对咽鳞癌Fadu细胞的肿瘤抑制作用及其机制。方法:利用CCK-8法检测TanⅡA联合DDP对Fadu细胞增殖抑制率的影响,流式细胞分析法检测细胞凋亡率变化及细胞周期分布情况,Western blotting法检测survivin、cleaved caspase-3及cleaved PARP蛋白的表达水平。结果:与丹参酮ⅡA和顺铂单独作用相比,两药联合作用于下咽鳞癌Fadu细胞72h后,细胞增殖受到明显抑制(均P<0.01),细胞周期阻滞于S期,凋亡细胞比例增加显著;caspase-3和PARP剪切段蛋白表达增多,survivin蛋白表达明显减少(P<0.01)。结论:丹参酮ⅡA联合顺铂具有协同抗咽鳞癌Fadu细胞的作用,其作用机制可能与下调凋亡抑制蛋白survivin的表达有关。Objective：The aim of this study is to investigate the inhibitory effect and mechanism of tanshinone Ⅱ A combined with cisplatin on tumor Fadu cells in pharyngeal squamous cell carcinoma. Method： Cytotoxicity was determined by CCK8 assay. Flow cytometry was used to detect apoptosis and cell cycle distribution. Western blot- ting was used to assess the protein expression of related signaling proteins. Result： Compared with the two single drug groups treated with Tan Ⅱ A and DDP respectively , the combination group showed significantly higher anti- proliferative rate（P〈0.01）, arrested cell cycle at S phase, and resulted in observably higher apoptotic cell frac- tions in human hypopharyngeal squamous cell carcinomas Fadu cells; Western blotting showed that the protein ex- pression of cleaved easpase 3 and cleaved PARP increased ,while survivin significantly decreased in the cells trea- ted with the combination of tanshinone IIA and cisplatin. Conclusion： Tanshinone Ⅱ A potentiates the efficacy of Cisplatin in Fadu cells, which may be attributed to the downregulation of survivin protein expression.

关 键 词：丹参酮ⅡA 顺铂 凋亡 Fadu细胞 

分 类 号：R739.6[医药卫生—肿瘤]