盐霉素联合顺铂对人胃癌细胞MKN-45增殖和凋亡的影响  

Effects of salinomycin combined with cisplatin on the proliferation and apoptosis of human gastric cancer cell line MKN-45

作　　者：赵明[1,2,3] 牛杰[1] 李芳芹[3] 王爱红[1,2] 庞秋霞[1,2] 陈美霓[1,2] 周丽珍[1] 赵菊梅[1,2] 

机构地区：[1]延安大学医学院,陕西延安716000 [2]延安市肿瘤防治研究重点研究室,陕西延安716000 [3]延安大学附属医院检验科,陕西延安716000

出　　处：《山东大学学报（医学版）》2017年第5期31-35,共5页Journal of Shandong University:Health Sciences

基　　金：国家大学生创新创业项目(2015 NO16956);延安大学研究生教育创新项目(YCX201637);延安大学校级青年项目(YDQ2016-30)

摘　　要：目的观察盐霉素联合顺铂对人胃癌细胞MKN-45增殖和凋亡的影响并探讨其作用机制。方法体外培养人胃癌细胞MKN-45至对数生长期,采用噻唑蓝(MTT)比色法分别检测盐霉素、顺铂及联合用药时细胞的增殖情况;吖啶橙/溴化乙锭(AO/EB)凋亡试剂盒荧光染色观察细胞凋亡情况;Western blotting法检测NF-κB、caspase-3蛋白表达水平。结果 MTT法显示盐霉素、顺铂均可抑制细胞增殖(P<0.05),联合给药比单药抑制效果更为显著(P<0.05);AO/EB荧光染色可见盐霉素、顺铂分别单药给药后,与对照组相比细胞凋亡形态明显,两药联合凋亡效果更为显著;Western blotting结果显示联合组与单药组和对照组相比NF-κB蛋白表达下降(P<0.01),caspase-3蛋白表达升高(P<0.01)。结论盐霉素可抑制胃癌细胞增殖,与顺铂联合应用可增强顺铂对人胃癌细胞MKN-45的抑制作用,其机制可能为抑制NF-κB活化,上调下游凋亡基因caspase-3的表达。Objective To investigate the effects and mechanisms of salinomycin （Sal） combined with cisplatin on the proliferation and apoptosis of human gastric cancer cell line MKN-45. Methods MKN-45 cells were cultured in vitro to logarithmic growth phase, and the proliferation rate of cell line MKN-45 preprocessed separately and jointly by Sal and cisplatin was detected with methyl thiazolyltetrazolium （MTT） method. The apoptosis were examined by AO/EB apoptosis kit and the protein expression levels of NF-κB and caspase-3 were detected with Western blotting. Results MTT assay indicated that the apoptosis rate of MKN-45 cells treated by the combination by Sal and cisplatin was obvi- ously more than that by Sal and cisplatin used separately （ P 〈 0.05 ）. AO/EB showed that administration of Sal and cisplatin respectively the apoptotic morphology was obvious compared with that of the control group, and the combined effect of the two drugs was more significant. Western blotting result showed that, after the Sal and cisplatin used in combination, the protein of NF-κB expression level decreased （ P 〈 0.05 ） and the protein caspase-3 increased （ P 〈0.05 ）. Conclusion Sal suppresses gastric cancer cell proliferation, and combination of Sal with cisplatin would enhance the effect cisplatin does. The mechanism may be inhibiting the activation of NF-κB and up-regulation of the caspase-3 apoptotic gene in the downstream.

关 键 词：盐霉素 顺铂 MKN-45 NF-ΚB CASPASE-3 

分 类 号：R735.2[医药卫生—肿瘤]