Protective Effect of Holothurian Intestine Against Indomethacin Induced Gastric Mucosal Damage in Rats  

作　　者：LI Xiaoyu QIAO Xuejing ZHANG Cuiping GAO Hua NIU Qinghui WU Tong ZHANG Qi TIAN Zibin 

机构地区：[1]Department of Gastroenterology,the Affiliated Hospital of Qingdao University [2]Department of Pharmacy,College of Medicine of Qingdao University

出　　处：《Journal of Ocean University of China》2017年第3期547-554,共8页中国海洋大学学报（英文版）

基　　金：supported by the Pharmacology Laboratory of Qingdao University;funding from the Dalian Bangchui Island Seafood Co., Ltd.;Shandong Medical and Health Science and Technology Development Plan (No. 2013WS0270);Natural and Science Funding of Shandong Province (ZR2014HM094);College and University Scientific Research Development Program of Shandong Province (J15LL5T);Qingdao People's Livelihood Science and Technology Plan (14-2-3-8-nsh)

摘　　要：Our study aimed to investigate the protective effects of Holothurian intestines(HI) on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin(IDM, 30 mg kg^(-1)). The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI(0.4g kg^(-1) d-1, 0.8 g kg^(-1) d^(-1) and 1.6 g kg^(-1) d^(-1)) prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2(PGE2) and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction(q PCR). Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines(IL-1β, IL-17, TNF-α) and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors(TGF-β, GSH, SOD activity and PGE2) were significantly elevated especially in the group with HI 1.61 g kg^(-1) d^(-1)(P < 0.05). Furthermore, the expression of COX-2 mRNA decreased significantly in HI groups(P < 0.05). The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage.Our study aimed to investigate the protective effects of Holothurian intestines（HI） on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin（IDM, 30 mg kg^-1）. The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI（0.4g kg^-1 d^-1, 0.8 g kg^-1 d^-1 and 1.6 g kg^-1 d^-1） prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2（PGE2） and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction（q PCR）. Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines（IL-1β, IL-17, TNF-α） and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors（TGF-β, GSH, SOD activity and PGE2） were significantly elevated especially in the group with HI 1.61 g kg^-1 d^-1（P 〈 0.05）. Furthermore, the expression of COX-2 mRNA decreased significantly in HI groups（P 〈 0.05）. The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage.

关 键 词：holothurian intestine NSAID INDOMETHACIN gastric ulcer sucralfate 

分 类 号：R965[医药卫生—药理学]