柿属植物叶绿体蛋白质编码基因密码子用法  

Analysis on codon usage in the chloroplast protein-coding genes of Diospyros spp.

作　　者：傅建敏[1,2,3] 索玉静[2,3] 刘慧敏[2,3] 谭晓风[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004 [2]国家林业局泡桐研究开发中心,河南郑州450003 [3]中国林业科学研究院经济林研究开发中心,河南郑州450003

出　　处：《经济林研究》2017年第2期38-44,共7页Non-wood Forest Research

基　　金："十二五"科技计划课题"涩柿种质资源收集评价与新品种选育"(2013BAD14B0502)

摘　　要：为给后续柿属植物叶绿体基因工程研究提供基础,利用Codon W、CUSP、SPSS软件对5个柿属植物叶绿体基因组中的55条蛋白质编码基因序列进行密码子使用模式分析。结果显示,柿属植物叶绿体基因组密码子第3位碱基GC含量为26.9%~27.8%,有效密码子数在49.58~50.18之间,密码子偏好性较弱;柿叶绿体基因组不同位置的有效密码子数不同,取值范围为32.36~59.64;中性绘图分析和ENC-plot绘图分析结果表明,柿叶绿体基因组的密码子偏好性主要受选择的影响;通过高频密码子和高表达优越密码子分析相结合的方法,筛选出23个柿属植物最优密码子,且这些密码子主要以A和U碱基结尾。In order to provide basis for the follow-up studies on chloroplast genetic engineering in Diospyros spp., codon usages of 55 chloroplast genes in five Diospyros spp species were analyzed by using CodonW, CUSP and SPSS softwares. The results showed that the GC content in the third codon position of the chloroplast genomes in Diospyros spp. was ranged from 26.9% to 27.8%, and effective number of codons ranged from 49.58 to 50.18, which indicated that the codon bias was weak. Effective number of codons at different location of the D.kak/chloroplast genome was different, which ranged from 32.36 to 59.64. According to the results of neutrality plot analysis and ENC-plot analysis, codon bias in the chloroplast genome of D. kaki was mainly affected by selection pressure. In addition, 23 codons to be affirmed as the optimal codons by using analysis of high-frequency codons and the high expression codons, ending with A and U.

关 键 词：柿属植物 叶绿体 密码子偏好性 最优密码子 

分 类 号：S665.2[农业科学—果树学]