EphA2在结直肠癌细胞中的生物学功能及其信号调控机制  

Biological Functions and Regulatory Mechanisms of EphA2 in Colorectal Carcinoma

作　　者：菅志远[1] 沈先锋[1] 方程[2] 高义[1] 

机构地区：[1]十堰市太和医院(湖北医药学院附属医院)肝胆胰外科诊疗中心,湖北十堰442000 [2]武汉大学中南医院循证与转化医学中心,武汉430071

出　　处：《河南大学学报（医学版）》2017年第2期115-118,共4页Journal of Henan University:Medical Science

基　　金：湖北省教育厅科学研究计划重点项目(D20152012)

摘　　要：[目的]探讨EphA2在结直肠癌细胞中的生物学功能及其信号调控机制。[方法]qRT-PCR法检测EphA2在结直肠癌细胞系中的表达情况,筛选得到表达量较高细胞株系,利用si RNA沉默该细胞系中EphA2的表达,利用MTT、Transwell、Western blot、qRT-PCR法检测细胞生物学功能变化及Wnt/β-catenin信号通路相关基因及蛋白的表达变化。[结果]在人结肠癌细胞HCT116、HT29和SW480中筛选得到EphA2高表达细胞HCT116,构建载体EphA2-si RNA并成功转染细胞HCT116。MTT、Transwell法检测结果显示,与对照组相比,EphA2-si RNA组中细胞的增殖、侵袭和迁移能力明显受到抑制,差异具有统计学意义(P<0.05)。qRT-PCR和western blot分析表明,与对照组相比,EphA2-si RNA组中Wnt/β-catenin信号通路相关蛋白E-cadherin、TCF-4、β-catenin和p-GSK-3βSer9的表达量均呈现下降趋势,差异具有统计学意义(P<0.05)。[结论]结直肠癌细胞中EphA2的表达与结直肠癌的进展和侵袭转移密切相关,EphA2可能通过调控Wnt/β-catenin信号通路影响结直肠癌的进展。[Objective]To investigate the biological function and mechanism of EphA2 in colorectal cancer. [Methods）The expression of EphA2 in colorectal cancer cell lines were detected by qRT-PCR, screened one cell lines with higher EphA2 expression. Silenced the expression of EphA2 in the cell line which we screened by siRNA technology. [Results）The results of MTT and Transwell analysis showed that inhibition of the EphA2 expression could suppress the proliferation, invasion and metastasis of the colorectal carcinoma cells （ all P 〈 0.05）. According to qRT PCR and western blot, the expression of related crucial protein of Wnt/β catenin pathway, including E-cadherin, TCF-4 and β catenin, and p-GSK-3βSer9 were significant decreased in the EphA2-siRNA group （P 〈 0.05 for all）. [Conclusion]EphA2 was involved in the development of colorectal carcinoma, and it might participate the tumor progression mediated by Wnt/β catenin pathway.

关 键 词：EPHA2 Wnt/β—catenin信号通路 结直肠癌 

分 类 号：R735.3[医药卫生—肿瘤]