外源脯氨酸对镍胁迫下小麦幼苗生长的影响  

Effect of Exogenous Proline on the Growth of Wheat Seedling under Nickel Stress

作　　者：巴青松[1] 张根生[1] 张典典 李桂萍[1] 宋运贤[1] 陶兴魁[2] 胡玉玲[1] 傅兆麟[1] 

机构地区：[1]淮北师范大学生命科学学院/资源植物生物学安徽省重点实验室,安徽淮北235000 [2]宿州学院生物与食品工程学院,安徽宿州234000

出　　处：《麦类作物学报》2017年第5期700-704,共5页Journal of Triticeae Crops

基　　金：安徽省高等学校省级自然科学研究项目(KJ2014B19;KJ2016A624;KJ2013A232);淮北师范大学博士科研启动基金项目(15600971);宿州学院特色种植业苗种生产工程技术研究中心开放课题(2014XKF19);安徽省自然科学基金项目(1708085QC62);安徽省大学生创新创业计划项目(201610373081)

摘　　要：为探究外源脯氨酸对镍胁迫下小麦根系生长发育的影响,以小麦品种轮选988为材料,采用溶液培养法,研究了不同浓度的脯氨酸对10μmol·L^(-1) Ni^(2+)胁迫下小麦幼苗株高和根系生长的影响。结果发现,镍胁迫严重抑制小麦生长;而外施脯氨酸对小麦幼苗的株高和根系生长的各项指标均有正向效应,以外施5mmol·L^(-1)脯氨酸的效果最好。与单独Ni^(2+)胁迫相比,外施5mmol·L^(-1)脯氨酸后,小麦幼苗根长、株高、根鲜重和根干重分别上升了24.8%、12.5%、16.0%和20.0%;超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)活性分别升高了54.30%、43.00%、13.08%、67.67%;超氧阴离子(O_2^-·)自由基、过氧化氢(H_2O_2)、丙二醛(MDA)含量分别下降了56.65%、34.54%、32.35%;渗透调节物质游离脯氨酸(Pro)和可溶性糖的含量均显著下降;根系活力上升了233.83%。外源脯氨酸可以显著提高小麦根的抗氧化能力,进而增强小麦的抗逆性,缓解镍对小麦根系的毒害作用。To study the effect of proline on nickel（Ni2＋） toxicity in wheat （Triticum aestivum L. ）, wheat seedlings exposed to 10 μmol · L^-1 NiSO4 were treated with different concentration proline. Results showed that Ni2＋ exposure depressed wheat growth. Exogenous proline have positve effects to plant height, root index of wheat seedings, with 5 mmol· L^-1 proline works best. Compared with the single Ni2＋ stress, after 5 mmol · L^-1 proline application, the root length, plant height, root fresh weight and root dry weight of the seedlings were significantly increased by 24.8 %, 12.5 %, 16.0 %and 20.0%, respectively. And the activity of superoxide dismutase （SOD）, peroxidase （POD）, ascorbate peroxidase（APX）, and catalase（CAT） were increased by 54. 30%, 43. 00%, 13. 08%, 67. 67%. The contents of superoxide anion （OF）, hydrogen peroxide （ H2O2 ）, and malondialdehyde （MDA）, signifi- cantly decreased by 56.65%, 34.54%, 32.35%, respectively. Meanwhile, the contents of proline and soluble sugar were decreased significantly. Ultimately, root activity was increased by 233.83 %. Therefore,exogenous proline can significantly improve the antioxidant capacity of wheat roots, and then it can enhance the resistance ability of wheat and alleviate the toxic effect of nickel on wheat.

关 键 词：脯氨酸 镍胁迫 活性氧代谢 小麦 

分 类 号：S512.1[农业科学—作物学] S311