氯霉素ELISA可视化微阵列芯片检测试剂盒评价研究  

Evaluation techniques of detecting chloramphenicol by ELISA-Based visualization microarray chip

作　　者：杜兵耀 文芳[2] 臧长江[1] 邵伟[1] 李昊[1] 张养东[2] 杨开伦[1] 余雄[1] 姜金斗 巩军 

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]农业部乳品质量监督检验测试中心(哈尔滨),哈尔滨150090

出　　处：《中国乳品工业》2017年第5期47-50,共4页China Dairy Industry

基　　金：国家现代农业产业技术体系建设专项(nycytx-04-01)

摘　　要：为了了解氯霉素ELISA可视化微阵列芯片检测试剂盒的有效性和可靠性,从灵敏度、特异性、准确度、可重复性、检出限、耐变性、批间变异等方面进行了评价,结果表明该试剂盒在测试范围内线性回归良好,相关系数R2可达0.9943,与结构相似的甲砜霉素、氟甲砜霉素的交叉反应率均小于1%,批间变异系数小于20%,牛奶中的平均回收率为108.5%,检出限为0.067μg/L,该试剂盒有较好的准确性、特异性与重复性,耐变性与批间变异均在可接受范围内,可用于牛奶样品中氯霉素的残留检测。In order to understand the effectiveness and reliability of domestic Chloramphenicol ELISA-based visualization microarray chip kit,evaluated in terms of sensitivity, specificity, accuracy, reproducibility, the detection limit, stability and inter-assay coefficients of variation. The results showed that the kit had a regression line of and correlation coefficient 0.9943. It showed less than 1% cross-reactivity with thiamphenicol and florfenicol, which was similar to chloramphenicol in structure. The inter-assay coefficients of variation is less than 20%, and the rate of recovery for chloramphenicol from milk was 108.5%, the corresponding the detection limit was 0.067 μg/L,This kit had a good accuracy, speci city and reproducibility, its stability and inter-assay were both in the acceptable range. This kit can be used in melamine detection from milk and milk powder.

关 键 词：氯霉素 ELISA 试剂盒 评价 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]