肥胖伴牙周感染小鼠体内B-1a细胞的表达初探  

A tentative exploration of the expression of B-1a cells in mice with obesity and periodontal infection

作　　者：王轶雄[1,2] 余挺[3] 谢宝仪[1] 轩东英[1] 章锦才[1] 

机构地区：[1]南方医科大学附属广东省口腔医院,广州510280 [2]南方医科大学口腔医院 [3]广州医科大学附属口腔医院.广州口腔疾病研究所.口腔医学重点实验室

出　　处：《实用口腔医学杂志》2017年第3期287-291,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81371151;81470750)

摘　　要：目的:分析肥胖伴牙周感染小鼠体内B-1a细胞的表达变化。方法:建立饮食诱导型肥胖伴实验性牙周感染的小鼠模型,通过免疫组化和蛋白质印迹的方法检测小鼠颌骨和脾脏中CD5、抗Ⅰ型胶原(Col-Ⅰ)抗体、IL-10的蛋白表达量;实时定量PCR检测小鼠颌骨中CD5、抗Col-Ⅰ抗体、IL-10的mRNA表达量。结果:牙周炎组颌骨中的CD5、IL-10、抗Col-Ⅰ抗体的蛋白和mRNA表达量均显著高于对照组(P<0.001);肥胖组脾脏中的CD5、IL-10、抗Col-Ⅰ抗体的蛋白表达量显著高于标准组(P<0.05);标准伴牙周真性结扎组脾脏中抗Col-Ⅰ抗体的表达量显著高于标准伴牙周假性结扎组(P<0.05)。结论:B-1a细胞在肥胖与牙周炎的炎症早期被激活并存在一定的病理意义,这两种炎症状态并没有在病理机制上出现交叉影响。Objective：To study the expression of B-1a cells in mice with obesity and periodontal infection.Methods：Mouse models of diet induced obesity combined with experimental periodontitis were established,the expression of CD5 protein,anti-collagenⅠ antibody（anti-Col-Ⅰantibody） and IL-10 protein was examined in mouse jaw bone and spleen by immunohistochemistry and Western blot;The mRNA expression of CD5,anti-Col-Ⅰantibody and IL-10 in mouse jaw bone was detected by real time quantitative PCR.Results：The mRNA and protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in jaw bone in periodontitis group were significantly higher than those in control group（P〈0.001）.The protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in spleen in obesity group were significantly higher than those in standard group（P〈0.05）.The protein expression of anti-Col-Ⅰantibody in spleen in standard accompanying periodontal ligature group was significantly higher than that in standard without periodontal ligature group（P〈0.05）.Conclusion：B-1a cells are activated in the early stage of obesity and periodontal inflammation with a certain pathological significance and without interation between the two inflammatory states in the pathological mechanism.

关 键 词：牙周炎 肥胖 颌骨 脾脏 B-1a细胞 

分 类 号：R781.4[医药卫生—口腔医学]