B类Ⅱ型清道夫受体在牙龈卟啉单胞菌影响泡沫细胞形成中的作用  

The role of SR-B Ⅱ in the oxidized low density lipid induced foam cell formation promoted by porphyromonas gingivalis

作　　者：贺晓丽[1] 梁冬雨[2] 刘峰[3] 陈建霞[3] 罗礼君[2,4] 

机构地区：[1]潍坊医学院,261053 [2]上海健康医学院附属嘉定区中心医院口腔科 [3]上海市结核病重点实验室,同济大学医学院附属上海市肺科医院 [4]同济大学附属口腔医院牙周病科

出　　处：《实用口腔医学杂志》2017年第3期306-311,共6页Journal of Practical Stomatology

基　　金：上海市科委自然基金(编号:13ZR1435600)

摘　　要：目的:观察牙龈卟啉单胞菌(P.g)诱导泡沫细胞形成过程中B类Ⅱ型清道夫受体(SR-BⅡ)的表达变化,并初步探讨其相关的作用机制。方法:用P.g和氧化型低密度脂蛋白(ox-LDL)共同刺激小鼠腹腔巨噬细胞,观察泡沫细胞的形成。采用Western blot和RT-PCR法检测泡沫细胞形成过程中SR-BⅡ表达含量的变化。用siRNA沉默SR-BⅡ的表达后检测泡沫细胞的形成情况。结果:P.g以及ox-LDL刺激小鼠腹腔巨噬细胞后,油红O染色观察到泡沫细胞的形成明显增多。在泡沫细胞形成过程中,P.g可以诱导SR-BⅡ表达增加,采用siRNA干扰SR-BⅡ表达后,P.g对泡沫细胞形成的促进作用减弱。结论:P.g可以促进SR-BⅡ的表达,调控SR-BⅡ表达可以改变泡沫细胞的形成。Objective：To investigate the role of scavenger receptor BⅡ（SR-BⅡ） in the oxidized low density lipid（ox-LDL） induced foam cells formation promoted by porphyromonas gingivalis（P.g）.Methods：Peritoneal macrophages were stimulated with P.g and ox-LDL,and then foam cells formation were checked.The expression of SR-BⅡ was detected by Western blot and real-time PCR.Next,siRNA targeting SR-BⅡ was used to detect the change of foam cells formation.Results：After being stimulated with P.g and ox-LDL,the foam cells formation was significantly increased.During the process of foam cells formation,P.g infection increased the expression of SR-BⅡ.And the knockdown of SR-BⅡ by siRNA significantly reduced the foam cells formation.Conclusion：P.g infection can increase the expression of SR-BⅡ and the regulation of SR-BⅡ expression can change the foam cells formation.

关 键 词：牙龈卟啉单胞菌 B类Ⅱ型清道夫受体(SR-BⅡ) 泡沫细胞 氧化型低密度脂蛋白(ox-LDL) 

分 类 号：R781.4[医药卫生—口腔医学]