GSK-3β对正畸牙齿移动的影响  

The effects of GSK-3β on orthodontic tooth movement

作　　者：孙聪[1] 刘文佳[2] 金岩[2] 金钫[1] 

机构地区：[1]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学口腔正畸科,西安710032 [2]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学组织工程中心,西安710032

出　　处：《实用口腔医学杂志》2017年第3期377-380,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81171001)

摘　　要：目的:观察GSK-3β对正畸牙齿移动距离的影响。方法:分别选30只野生C57小鼠和20只GSK-3β基因敲除C57小鼠。野生型和基因敲除型各20只,正畸加力3、5、7、14 d(5只/组)后处死,取材固定上颌骨扫描Micro CT测量牙齿移动距离,冰冻切片免疫荧光染色观察破骨情况。野生型10只腹腔注射LiCl(隔天100 ng/ml)加力3 d,7 d处死后扫描Micro CT。结果:与野生型组同期结果相比,GSK-3β基因敲除小鼠牙齿移动距离降低(P<0.05),压力侧破骨细胞减少(P<0.05);基因敲除组与野生型注射LiCl组牙齿移动距离无统计学差异。结论:GSK-3β可以影响破骨细胞形成从而影响正畸牙齿移动。Objective：To observe the effects of GSK-3β on orthodontic tooth movement.Methods：30 wild type C57 mice and 20 GSK-3β knockout C57 mice were used.Orthodontic force was applied in 20 wild-type and 20 GSK-3β knockout mice,3 d,5 d,7 d and 14 d（n=5） after treatment the mice were sacrificed,then the jaws were scanning by Micro CT,tooth movement distance was measured,frozen sections were examined by immunofluorescence staining.10 wild type mice were given intraperitoneal injection of LiCl （100 ng/ml every other day）,3 d and 7 d after orthodontic treatment the mice were sacrificed respectively and then the jaw bones were scanned Micro CT.Results：Compared with the wild type group at the same time,the tooth movement distance of GSK-3β gene knockout mice was significant reduced（P〈0.05）,and osteoclasts were decreased at the pressure side（P〈0.05）.There was no significant difference of tooth movement distance between wild type＋LiCl group and GSK-3β group.Conclusion：GSK-3β can affect osteoclast formation thus affecting orthodontic tooth movement.

关 键 词：GSK-3Β 正畸牙齿移动 破骨细胞 

分 类 号：R783.5[医药卫生—口腔医学]