羟考酮对大鼠单肺通气后急性肺损伤的影响  

Influence of Oxycodone on Acute Lung Injury Following One-lung Ventilation in Rats

作　　者：王开伟[1] 朱国松[1] 林洪启[1] 

机构地区：[1]郑州大学人民医院麻醉科,郑州450003

出　　处：《河南医学高等专科学校学报》2017年第2期107-110,共4页Journal of Henan Medical College

基　　金：河南省科技计划项目(122300410408)

摘　　要：目的探讨羟考酮对大鼠单肺通气后急性肺损伤的影响及机制。方法 32只成年清洁级SD大鼠随机分为4组:对照组、模型组、生理盐水组、羟考酮组,各8只。插管过深法建立大鼠急性肺损伤模型,取大鼠左肺上叶测量肺组织含水量;取大鼠部分左肺下叶进行HE染色,观察肺组织的病理学改变;酶联免疫吸附法(ELISA)检测炎症因子白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)和肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)等指标。结果光镜下观察HE染色后的肺组织,对照组肺组织形态结构基本正常,未见明显病理学改变;模型组和生理盐水组肺组织中可见大量渗出及炎性细胞浸润,肺间质增厚且充血明显,肺泡变小,肺实质增加显著;羟考酮组肺组织可观察到少量渗出及炎性细胞浸润,肺组织病理学改变较轻,轻度充血。与对照组相比较,模型组和生理盐水组肺组织的含水量、病理学评分,炎性因子IL-6、IL-1β和TNF-α等指标均上升,差异有统计学意义(P<0.05);与模型组相比较,羟考酮组肺组织的含水量、病理学评分,炎性因子IL-6、IL-1β和TNF-α等指标均下降明显,差异有统计学意义(P<0.05)。结论羟考酮能够抑制SD大鼠单肺通气后急性肺损伤,其机制可能与其减轻炎症反应有关。Objective To discuss the influence and mechanism of Oxycodone on acute lung injury following one-lung ventilation in rats. Methods Thirty-two adult male Sprangue-Dawley rats were ran-domly divided into four groups： control g roup, model group, saline group and Oxycodone group, with 8 rats each group. Acute lung injury model was esteblished with deep intubation; the water content in lung tissue was determined through taking the upper lobe of left lung ; HE was stained through taking the lower lobe of left lurg to abserve the pathological changes of lung t issue; inflammatory facotr, interleukine-6 , interleukin-1 （3 and tumor necrosis facotr-a were measured with anzyme linked immunosorbent assay. Re-sults After HE staining, in control group, there was no obvious pathological change of lung t is sue , oc-casionally there were inflammatory cells and exudates. In model group and saline group, there were a large number of inflammatory cell infiltration and alveolar exudates. Pulmonary interstitial was obviously hyperemia and hemorrhage. However, in Oxycodone Group, the pathological change of lung tissue were relieved significantly. Compared with control Group, in model Group and saline Group, the content of lung water increased significantly （P 〈0. 05） ; lung tissue pathological score increased significantly （P 〈0.05） ; the content of IL-6,IL-1（3 and TNF-a in lung tissue increased significantly （P 〈0.05）. Com-pared with model Group, in Oxycodone Group, the content of lung water decreased significantly （ P 〈 0.05） ; lung tissue pathological score decreased significantly （P〈0.05） ; the content of IL-6 ,IL-1 （Band TNF-a in lung tissue decreased significantly （ P 〈 0. 05 ） . Conclusion Oxycodone can alleviate acute lung injury induced by OLY, and the mechanism may be in evolve inhibition of inflammatory reaction.

关 键 词：急性肺损伤 羟考酮 单肺通气 炎症反应 

分 类 号：R364.11[医药卫生—病理学]