槲皮素对大肠杆菌和金黄色葡萄球菌的体外抑菌作用的研究  

In Vitro Bateriostasis of Qucercetin against Escherichia Coli and Staphlococcus Aureus

作　　者：洪志伟[1] 孟令宇[1] 葛雅琨[1] 张元新[1] 

机构地区：[1]吉林化工学院,生物与食品工程学院,吉林吉林132022

出　　处：《吉林化工学院学报》2017年第5期38-41,共4页Journal of Jilin Institute of Chemical Technology

摘　　要：槲皮素是普遍存在于植食性植物中的一类黄酮类化合物,具有明显的抗氧化、抗炎、保肝、抗肿瘤、抑菌等多种生物学功能特性.通过SDA-PAGE和碱性磷酸酶实验分析槲皮素作用前后的菌体蛋白质及碱性磷酸酶的变化,研究其对金黄色葡萄球菌和大肠杆菌的抑菌效果.研究结果表明,槲皮素是通过破坏细胞壁结构、抑制菌体对蛋白质的利用,导致细菌正常生理功能的丧失或减退,达到抑菌的目的.Quercetin, a kind of flavonolignans found in phytophagous plants, has obvious biological function, such as antioxidant,anti-inflammatory,anti-tumor and bacteriostatic properties.Though SDS-PAGE and alkaline phosphatase assay, we analyze the change of protein and activity of alkaline phosphatase after quercetin treatment of Escherichia coli and Staphylococcus aureus.The results show that quercetin inhibit the growth and protein utilization of the Escherichia coli through the disruption of cell wall and the loss of physiological function of bacterium.

关 键 词：槲皮素 抑菌作用 金黄色葡萄球菌 大肠杆菌 

分 类 号：O501[理学—物理]