氯化两面针碱对胃癌细胞株SGC7901凋亡的影响及初步机制  

Effect of nitidine chloride on cell apoptosis in gastric cancer cell line SGC7901

作　　者：张婉君 陈阳 张娜 

机构地区：[1]台州恩泽医疗中心(集团)路桥医院药剂科,浙江省台州市318050

出　　处：《世界华人消化杂志》2017年第14期1248-1254,共7页World Chinese Journal of Digestology

摘　　要：目的探讨氯化两面针碱对胃癌细胞株SGC7901凋亡的影响,并研究其可能的初步机制.方法MTT法检测不同浓度氯化两面针碱(0、5、10、20和40?mol/L)对胃癌细胞株SGC7901存活率的影响;Hoechst染色观察SGC7901细胞的形态学;Annexin V-FITC/PI双染法检测SGC7901细胞的凋亡;JC-1单标法检测细胞内线粒体膜电位变化;免疫印迹法检测caspase-3剪切体、caspase-9剪切体和PARP剪切体与其各自未激活体的蛋白表达变化.结果S G C 7 9 0 1的存活率随着氯化两面针碱浓度的增加而逐渐下降,并与时间呈正相关;Hoechst染色与Annexin V-FITC/PI双染法结果分别显示氯化两面针碱干预后SGC7901细胞的凋亡特征更显著,细胞凋亡率明显增多;经过氯化两面针碱处理后,SGC7901细胞线粒体膜电位显著下降,caspase-3剪切体、caspase-9剪切体和PARP剪切体蛋白表达水平显著上升,其未激活体蛋白表达明显下降.结论氯化两面针碱呈浓度依赖性抑制SGC7901细胞的存活率,增加细胞凋亡率,可能是通过激活内源性线粒体通路有关.AIM To investigate the effect of nitidine chloride on cell apoptosis in gastric cancer cell line SGC7901, and to explore the underlying mechanisms.METHODS After SGC7901 cells were treated with different concentrations of nitidine chloride （0, 5, 10, 20 or 40 ~tmol/L）, cell survival was determined by MTT assay, cell morphology was observed by Hoechst staining, cell apoptosis was measured by Annexin V-FITC/PI double staining, and mitochondrial membrane potential was examined by JC-1 staining. Furthermore, the expression of cleaved caspase-3, cleaved caspase-9, cleaved PARP and their respective un-cleaved protein was detected by Western blot.RESLIL TS Nitidine chloride decreased SGC7901 cell survival in a dose- and time-dependent manner. Hoechst and Annexin V-FITC/PI double staining showed increased apoptotic cells after nitidine chloride treatment. In addition, nitidine chloride treatment significantly increased the protein expression of cleaved caspase-3, cleaved caspase-9 and cleaved PARP, while the expression their respective un-cleaved protein was reduced.CONCLUSION Nitidine chloride can decrease SGC7901 cell survival and increase cell apoptosis possibly via inhibition of mitochondrial-dependent pathways.

关 键 词：氯化两面针碱 胃癌细胞株SGC7901 凋亡 线粒体 

分 类 号：R735.2[医药卫生—肿瘤]