舒尼替尼通过Wnt/β-catenin信号抑制肾癌细胞体外生长和侵袭的实验研究  

Sunitinib inhibits renal cancer cell growth in vitro and invasion through Wnt/β-catenin signaling pathway:An experimental study

作　　者：杨渝[1] 邵志强[1] 郭嘉祥 

机构地区：[1]解放军总医院第一附属医院泌尿外科,北京100048

出　　处：《海南医学院学报》2017年第8期1034-1036,1040,共4页Journal of Hainan Medical University

基　　金：北京市科技计划课题(Z151100004015194)~~

摘　　要：目的:研究舒尼替尼对肾癌细胞体外生长、侵袭及Wnt/β-catenin信号通路的影响。方法:培养肾癌细胞株ACHN并用不同剂量舒尼替尼(1、2、4、8μmol/L)进行处理,以不含舒尼替尼的处理条件作为阴性对照。处理后24h时,测定细胞中凋亡基因、侵袭基因、Wnt/β-catenin信号通路的mRNA表达量。结果:处理后24h时,1、2、4、8μmol/L舒尼替尼组中NPRL2、Bax、caspase-3、caspase-9的mRNA表达量显著高于阴性对照组,MMP2、MMP9、Vimentin、N-cadherin、Wnt、β-catenin的mRNA表达量显著低于阴性对照组;舒尼替尼剂量越高,细胞中NPRL2、Bax、caspase-3、caspase-9的mRNA表达量越高,MMP2、MMP9、Vimentin、N-cadherin、Wnt、β-catenin的mRNA表达量越低。结论:舒尼替尼能够通过抑制肾癌细胞中Wnt/β-catenin信号通路来增加凋亡基因的表达、抑制侵袭基因的表达,进而抑制细胞的生长和侵袭。Objective; To study the effect of sunitinib on renal cancer cell growth in vitro and invasion as well as Wnt/β- catenin signaling pathway. Methods： Renal cancer cell lines ACHN were cultured and processed with different doses of sunitinib （1 μmol/L, 2 μmol/L, 4 μmol/L and 8 μmol/L）, and sunitinib-free processing condition was used as negative control. Twenty-four hours after processing, the mRNA expression levels of apoptosis genes, invasion genes and Wnt/β-catenin signaling pathways in cells were detected. Results.. Twenty-four hours after treatment, NPRL2, Bax, caspase-3 and caspase-9 mRNA expression in 1 μmol/L, 2μmol/L, 4 /zmol/L and 8μmol/L sunitinib groups were significantly higher than those in negative control group while MMP2, MMPg, Vimentin, N-cadherin, Wnt and β-catenin mRNA expression were significantly lower than those in negative control group; the higher the dose of sunitinib, the higher the NPRL2, Bax, caspase-3 and caspase-9 mRNA expression while the lower the MMP2, MMPg, Vimentin, N-cadherin, Wnt and β-catenin mRNA expression in cells. Conclusions ： Sunitinib can inhibit the Wnt/β-catenin signaling pathway in renal cancer ceils to increase the expression of apoptosis genes, inhibit the expression of invasion genes and thereby inhibit the cell growth and invasion.

关 键 词：肾癌 舒尼替尼 WNT/Β-CATENIN 凋亡 侵袭 

分 类 号：R737.11[医药卫生—肿瘤]