灯盏花素对肝癌细胞HepG2和结肠癌细胞Caco2的体外抗肿瘤活性筛选  

Screening of in vitro anti-tumor activity of breviscapine on HepG2 and Caco2 cell lines

作　　者：曹昌娥[1] 赖泳[1] 杜一民[1] 

机构地区：[1]大理大学药学与化学学院药理学教研室,云南大理671000

出　　处：《中国现代医学杂志》2017年第10期1-5,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81241139);国家自然科学基金(No:81360511)

摘　　要：目的观察灯盏花素对人肝癌细胞(HepG2)和人结肠癌细胞(Caco2)的体外增殖抑制影响。方法以不同浓度的灯盏花素分别处理HepG2和Caco2细胞24、48和72 h后采用MTT法检测灯盏花素对2种细胞活力的影响,以半数抑制浓度(IC50)及治疗指数(TI)作为评价其抗肿瘤活性及安全性指标。结果灯盏花素对HepG2表现高浓度抑制作用,浓度为20 000μmol/L时,对HepG2细胞24、48和72 h的抑制率分别为37%、63%和69%;对Caco2细胞呈浓度依赖性,浓度为5 000μmol/L时,对Caco2细胞24、48和72 h的抑制率分别为72%、95%和92%。灯盏花素对HepG2 24h的IC50值为24320.5μmol/L;48h的IC50值为30 667.7μmol/L;72 h的IC50值为15 586.1μmol/L。对Caco2细胞24 h的IC50值为11 417.0μmol/L;48 h的IC50值为832.2μmol/L;72 h的IC50值为1 369.2μmol/L。结论灯盏花素对HepG2和Caco2细胞均有不同程度的抑制作用,对Caco2的抑制效果更明显。Objective To investigate the inhibitory effects of breviscapine on in vitro proliferation of human hepatocellular carcinoma cell line HepG2 and colon cancer cell line Caco2. Methods Both HepG2 and Caco2 cell lines were treated with breviscapine at different concentrations for 24, 48 and 72 h, and the inhibitory rate was analyzed by MTT assay. The 50% inhibitory concentration （IC50） and the therapeutic index （TI） were used as indicators to assess their anti-tumor activity and safety. Results Breviscapine at high concentration displayed inhibitory effect on HepG2; when the concentration was 20,000 μmol, its inhibitory rate to HepG2 cells after 24, 48 and 72 h was 37%, 63% and 69% respectively. As to Caco2 cells breviscapine showed concentration dependency; when the concentration was 5,000 μmol, its inhibitory rate after 24, 48 and 72 h was 72%, 95% and 92% respectively. The ICS0 of breviscapine on HepG2 cells after 24, 48 and 72 h was 24,320.5 μmol, 30,667.7 μmol and 15,586.1 μmol respectively;while that on Caco2 ceils after 24, 48 and 72 h was 11,417.0 μmol, 832.2 μmol and 1,369.2 μmol respectively. Conclusions Breviscapine exhibits different degree of inhibitory effect on HepG2 and Caco2 cells, the effect is more significant on Caco2 cells.

关 键 词：灯盏花素 HepG2细胞 Caco2细胞 MTT法 抗肿瘤活性 

分 类 号：R735.7[医药卫生—肿瘤] R735.3[医药卫生—临床医学]