去甲基化酶TET2在套细胞淋巴瘤中的表达及其作用机制  

Expression of TET2 mRNA and its role in mantle cell lymphoma

作　　者：刘小柳[1] 雷坚[2] 邹立新[1] 彭浪[1] 

机构地区：[1]长沙市第四医院血液肿瘤科,湖南长沙410006 [2]湖南省肿瘤医院病理科,湖南长沙410013

出　　处：《华夏医学》2017年第1期19-22,共4页Acta Medicinae Sinica

摘　　要：目的:探讨去甲基化酶TET2 mRNA在套细胞淋巴瘤(MCL)中的表达及其作用机制。方法:实时定量PCR检测TET2 mRNA表达,免疫比色法检测DNA中5-hm C水平,甲基化特异性PCR检测p15基因甲基化水平,并分析它们间的相互关系。结果:MCL患者TET2 mRNA表达水平及5-hm C含量均低于正常对照组,差异有统计学意义(P<0.01),TET2 mRNA水平与5-hm C含量呈正相关;MCL中p15基因甲基化率(5/20)高于对照组(0/10);MCL患者中,与p15未甲基化组比较,p15甲基化组的TET2 mRNA表达水平更低、5-hm C含量更低(P<0.05)。结论:TET2可能通过调节5-hm C生成,从而改变p15基因的甲基化态势,影响MCL的发生发展。Objective：To investigate the expression of demethylase TET2 mRNA and its role in mantle cell lymphoma（MCL）.Methods：The mRNA expression of TET2 was detected by real-time quantitative PCR,the level of 5-hm C in DNA was detected by immune colorimetric method,and the methylation of p15 gene was detected by methylation-specific PCR.Results：The levels of TET2 mRNA and 5-hm C in MCL patients were all significantly lower than those in controls（P 〈 0.01）,and TET2 mRNA level was positively correlated with the content of 5-hm C.The methylation rate of p15 gene in MCL（5/20） was higher than that in controls（0/10）.The expression of TET2 mRNA and the 5-hm C level in p15-methylation group were all lower than these in p15-unmethylated group（P 〈 0.05）.Conclusion：TET2 may change the methylation status of p15 by regulating the production of 5-hm C in MCL.

关 键 词：套细胞淋巴瘤 TET2 5-羟甲基化胞嘧啶 P15 甲基化 

分 类 号：R733[医药卫生—肿瘤]