两种磁性纳米颗粒(Fe_3O_4、Fe_3O_4@柠檬酸)的制备及其在DNA提取分离中的应用  

Preparation of two magnetic nanoparticles (Fe_3O_4,Fe_3O_4@citric acid) and application in DNA separation and extraction

作　　者：王飞[1] 张璇 柯长洪[2] 李海洋[1] 马家骅[1] 穆洪涛[3] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621000 [2]广东顺德创新设计研究院,佛山528311 [3]生物与食品工程学院广东第二师范学院,广州510303

出　　处：《化工新型材料》2017年第5期188-190,共3页New Chemical Materials

基　　金：国家自然科学基金(30902005)

摘　　要：采用溶剂热法制备表面修饰柠檬酸的磁性Fe_3O_4纳米粒子和磁性Fe_3O_4纳米粒子,并对其粒径大小、晶体结构和磁性能进行表征,并考察其用于DNA提取分离的效果。结果表明,两产物均为立方晶系的Fe_3O_4纳米颗粒。磁性Fe_3O_4纳米粒子和表面修饰柠檬酸的磁性Fe_3O_4纳米粒子的平均粒径为411.1nm和586.3nm。当全血体积200μL、磁性纳米粒子用量2.0mg时,提取的DNA浓度最高分别为270.6ng/μL(Fe_3O_4)和466.4ng/μL(Fe_3O_4@柠檬酸)。Fe304 nanoparticles and citric acid capped magnetic Fe3O4 nanoparticles were prepared by solvothermal method. The diameter size, crystal structure and magnetism of nanoparticles were characterized. The effects of their applica tions in extracting DNA from whole blood were studied. The results indicated that both products were cubic crystal Fe3O4, nanopartic,es and their average particle sizes were 411. lnm （Fe3O4） and 586.3 nm （Fe3O4, citric acid）, respectively. When the whole blood volume was 200/μL and the amount of magnetic beads was 2mg, the highest DNA concentration were 270.6ng//H.（Fe3O4） and 466.4ng/μL （Fe3O4 citric acid）, respectively.

关 键 词：FE3O4 溶剂热法 柠檬酸 磁性纳米颗粒 DNA提取 

分 类 号：Q78[生物学—分子生物学] TB383.1[一般工业技术—材料科学与工程]