替格瑞洛含药血清对人胃黏膜上皮细胞GES-1的损伤作用  

Effects of ticagrelor on human gastric mucosal epithelial GES - 1 cells

作　　者：樊叶[1] 韩维维[1] 何帮顺[2] 张振玉[1] 

机构地区：[1]南京医科大学附属南京医院消化内科,江苏 南京210006 [2]南京医科大学附属南京医院中心实验室

出　　处：《徐州医科大学学报》2017年第4期252-255,共4页Journal of Xuzhou Medical University

摘　　要：目的探讨替格瑞洛含药血清对人胃黏膜上皮细胞株GES-1的损伤作用。方法分离1例服用替格瑞洛（180mg／d）后2h的患者血清，并配制成不同浓度（5％、10％、15％、20％）的含药血清，同时取该患者服用替格瑞洛前的血清作为对照，培养GES-1细胞24、48、72h。采用MTT法检测细胞增殖，倒置相差显微镜观察各浓度组含替格瑞洛血清培养GES-1细胞后细胞形态学变化，流式细胞仪检测各浓度组细胞凋亡情况。结果MTr显示替格瑞洛含药血清对GES-1细胞的损伤随着浓度增加而增强，呈浓度依赖性（P〈0．05），而无时间依赖性（P〉0．05）。光镜下可见含药血清组的贴壁细胞数量减少，细胞变圆、悬浮，部分细胞核浓缩，细胞损伤随着含药血清浓度增加而加重。流式细胞术显示，替格瑞洛含药血清组细胞凋亡率增大，且随浓度增加而增强。结论替格瑞洛含药血清对人胃黏膜上皮细胞有损伤作用．Objective To investigate the effects of ticagrelor on human gastric mucosal epithelial GES - 1 cells. Methods Serum was collected from a patient taking 180 mg/d ticagrelor 2 h after treatment, and various concentrations （5% , 10% , 15% , and 20% ） of ticagrelor in serum were prepared. Meanwhile, the serum separated from the same pa- tient before ticagrelor treatment was taken as a control. GES - 1 cells were incubated with the ticagrelor - containing ser- um for 24, 48 and 72 h. The proliferation rate of GES - 1 cells was examined using MTF assay. The morphological chan- ges of GES - 1 cells treated with different concentrations of ticagrelor for 24 h were observed under an inverted phase con- trast microscope. Flow cytometry was used to detect cell apoptosis after GES - 1 cells incubated with the above serum. Results According to MTI＂ assay, ticagrelor inhibited the proliferation of GES - 1 cells in a concentration - dependent manner （P 〈 0.05） , rather than in a time - dependent manner （P 〉 0.05 ）. Compared with the control group, the ti- cagrelor - containing serum group showed a less number of adherent cells, where the cells became round and suspended, some cells presented condensed nucleus and cellular damages gradually worsen as the concentration of ticagrelor was in- creased. According to flow cytometry, the apoptosis rate of GES - 1 cells was increased in a dose dependent manner ＇after treated with ticagrelor. Conclusion Ticagrelor can result in damages in human gastric mucosal epithelial GES - 1 cells.

关 键 词：替格瑞洛 人胃黏膜上皮细胞 血清药理学 细胞增殖 细胞凋亡 

分 类 号：R573.3[医药卫生—消化系统]