肾缺血／再灌注损伤中近曲小管Legumain表达的研究  

Expression of legumain in the proximal tubule after renal ischemia/reperfusion injury

作　　者：高帅[1] 夏安周[2] 侯筱宇 郭义峰[1] 

机构地区：[1]上海交通大学附属第一人民医院泌尿外科,上海200080 [2]徐州医科大学药理学教研室,江苏徐州221004 [3]徐州医科大学基础医学院生物化学与分子生物学研究中心

出　　处：《徐州医科大学学报》2017年第5期281-286,共6页Journal of Xuzhou Medical University

基　　金：国家自然科学基金（81370842）;江苏省脑病生物信息重点实验室开放课题（1503）

摘　　要：目的建立小鼠肾缺血／再灌注损伤模型，观察小鼠肾缺血／再灌注后的损伤、Legumain的表达，探讨Legumain在小鼠肾脏缺血／再灌注损伤中的作用及可能机制。方法30只C57BL／6小鼠行右肾切除后随机分为缺血／再灌注损伤组（IRI组）、对照组，夹闭左肾蒂45min缺血，缺血后再灌注不同时间（1h、6h、24h），检测各组小鼠血肌酐、尿素氮水平，观察肾组织形态学变化，免疫组化技术与蛋白印迹分析Legumain的表达。结果IRI组肾脏经过缺血／再灌注后，血肌酐及尿素氮水平在1h、6h、24h再灌注时间点较对照组明显升高（P〈0．05）。IRI组小鼠肾损伤程度较对照组明显加重，表现为肾小管细胞坏死脱落、间质水肿、炎症细胞浸润增多，损伤随着再灌注时间的延长而加重。IRI组肾近曲小管中Legumain的表达较对照组明显增强（P〈0．01）。结论肾缺血后再灌注会造成损伤，导致肾功能下降、肾小管细胞损伤坏死、Legumain表达增强，Legumain的表达变化可能在肾缺血／再灌注损伤中起重要作用。Objective To establish a mouse model of renal ischemia/reperfusion injury, and investigate the renal ischemia/reperfusion injury, the expression and role of legumain in mice with renal ischemia/reperfusion injury and pos- sible mechanisms. Methods A total of 30 C57BL/6 mice were randomly divided into two groups after right renal resection： an ischemia/reperfusion injury （IRI） group and a control group. The mice were subject to blockage of the left renal pedicle for 45 rain followed by reperfusion for various times （ 1,6 and 24 h）. Then, the levels of serum creatinine and urea nitrogen and morphological changes of each group were observed, while the expression of legumain was examined by immunohistochemistry and western blotting. Results After renal ischemia/reperfusion, the IRI group produced remarkably higher levels of serum creatinine and urea nitrogen than the control group after reperfusion for 1,6, and 24 h （ P 〈 0.05 ）. Compared with the control group, the IRI group presented markedly worsened renal injury which was character- ized in necrosis of the renal tubular cells, interstitial edema, and increased infiltration of inflammatory cells, and aggravated as reperfusion extended. The expression of legumainin in the proximal tubule of the IRI group was significantly higher than that in the control group （P 〈 0.01 ）. Conclusions Renal ischemia/reperfusion can lead to decreased renal function, renal tubular damage and higher expression of legumain. The change in the expression of legumain may play an im- portant role in ischemia/reperfusion injury.

关 键 词：肾脏 缺血/再灌注损伤 LEGUMAIN 

分 类 号：R692[医药卫生—泌尿科学]