甘薯IbFBA5基因的时空表达及与碳水化合物积累的关系  

Temporal and spatial expression of IbFBA5 gene and its relationship with carbohydrate accumulation in sweetpotato(Ipomoea batatas)

作　　者：苏在兴 高闰飞 唐维[1,2] 闫会[1,2] 后猛[1,2] 王欣[1,2] 刘亚菊[1,2] 张允刚[1,2] 马代夫[1,2] 李强[1,2] 

机构地区：[1]江苏徐淮地区徐州农业科学研究所/农业部甘薯生物学与遗传育种重点实验室/江苏徐州甘薯研究中心,江苏徐州221131 [2]中国农业科学院甘薯研究所,江苏徐州221131

出　　处：《植物生理学报》2017年第5期857-864,共8页Plant Physiology Journal

基　　金：现代农业产业技术体系建设专项资金(CARS-11;甘薯);国家高技术研究发展计划(2012AA101204);江苏省重点研发计划项目(BE2015313)~~

摘　　要：果糖-1,6-二磷酸醛缩酶(FBA)是糖酵解、糖异生、磷酸戊糖途径及光合作用过程的关键酶。本研究利用荧光定量分析研究 IbFBA5基因在甘薯不同组织及不同生长时期的表达及与叶片和贮藏根中碳水化合物积累的关系。结果表明, IbFBA5具有组织表达特异性,在甘薯‘徐781’叶片中表达量最高,其次是地下部(牛蒡根、储藏根和纤维根),叶柄、茎和花中表达量较低;甘薯储藏根初始膨大期至膨大盛期, IbFBA5在‘徐薯18’叶片中表达量均极显著高于在‘徐781’中的表达量;而在储藏根初始膨大期和膨大盛期,该基因在‘徐781’储藏根中的表达量均显著或极显著高于‘徐薯18’中的表达量。可溶性糖和淀粉含量分析表明,甘薯初始膨大期至膨大中期,叶片和储藏根中果糖和葡萄糖的含量均极显著提高,储藏根中淀粉含量也极显著提高,然而储藏根中的蔗糖含量及叶片中淀粉含量极显著降低。甘薯叶片和储藏根中 IbFBA5的表达模式分别与不同时期叶片中淀粉的含量变化和储藏根中果糖及葡萄糖的含量变化趋势具有一致性。Fructose-1,6-bisphosphate aldolase （FBA） is a key enzyme in the pathway of glycolysis, gluconeo- genesis, pentose phosphate and photosynthesis. The expression of IbFBA5 in different tissues and different growth periods were carried out by qRT-PCR analysis in this paper. We also analyzed the relationship among the gene expression levels and carbohydrate accumulations in leaves and storage roots. The results indicated that the expressions oflbFBA5 were specific in different tissues, which showed highest expression in leaves and also high expression in roots, but low expression in petiole, stems and flowers. The gene expression levels were discrepant in leaves and roots of different genotypes, ＇Xushul 8＇ and ＇Xu781＇, respectively. The expression lev- els were significantly higher in the leaves of ＇Xushu 18＇ than that of ＇Xu781＇ from initial expansion stage to peak expansion stage of storage roots. The expression levels were also significantly higher in the storage roots of ＇Xu781＇ than that of ＇Xu 18＇ at initial period and peak period of expansion respectively. The contents of fruc- tose and glucose were increased obviously from initianl period to medium period in leaves and storage roots as well as the starch contents in storage roots. On the contrary, the sugar contents in storage roots and starch con- tents in leaves were depressed sharply at this period. We found that the expression patterns oflbFBA5 had con- sistency with the trends of starch contents in leaves and fructose and glucose contents in storage roots respec- tively.

关 键 词：甘薯 果糖-1 6-二磷酸醛缩酶 时空表达 可溶性糖 淀粉 

分 类 号：S531[农业科学—作物学]