急性肝衰竭时小鼠上消化道及胆汁菌群的变化  

Microflora changes in upper digestive tract and bile in acute liver failure mice

作　　者：刘华娟[1] 余蕾[2] 胡亚欣[1] 程明亮[3] 

机构地区：[1]贵州医科大学临床医学院,550004 [2]贵州医科大学附属医院检验科 [3]贵州医科大学附属医院感染科

出　　处：《中华医学杂志》2017年第21期1664-1667,共4页National Medical Journal of China

基　　金：贵州省科技合作项目（黔科合LH字[2016]7243号）

摘　　要：目的 研究急性肝衰竭时小鼠上消化道和胆汁内菌群的变化。方法 实验分为D－氨基半乳糖建立的肝衰竭模型组（M组）和正常对照组，采集小鼠口腔、胃、十二指肠盥洗液、胆汁，高通量测序技术测定细菌16 S rDNA的V3＋V4区，得出细菌丰度及多样性信息；LEfSe （LDA effect size）分析找出肝衰竭前后各段的差异菌。结果 门水平上，M组小鼠口腔和胃内TM7的相对丰度高于正常对照组（P〈0.05）。科水平上，与正常对照组相比，M组胃内巴斯德氏菌科（Pasteurellaceae）减少（P〈0.05），胆汁内乳杆菌科（Lactobacillaceae）增多（P〈0.05）。结论 急性肝衰竭时小鼠上消化道及胆汁内菌群有显著改变。Objective To study the changes of upper digestive tract and bile flora associated with acute liver failure in mice. Methods Mice were randomly divided into 2 groups： control group and acute liver failure group （ group M）. Acute liver failure in group M was induced by intraperitoneal injection of D- galactosamine （D-GaIN）. The information of relative abundance and diversity were observed by high- throughput sequencing of V3 ＋ V4 region in 16 S rDNA from bacteria of oral cavity, stomach, duodenal and bile. Bacterial translocation and changes associated with acute liver injury were identified by LEfSe （ LDA effect size） analysis. Results At the phylum level, compared with control group, the relative abundance of TM7 in oral cavity and stomach increased in group M. At the family level, compared with control group, group M resulted in a significant decrease in Pasteurellaceae in the stomach （ P 〈 0. 05 ） and a increase in Lactobacillaceae in the bile （P 〈 0. 05 ）. Conclusion The diversity of bacteria and relative abundance of each bacterium in upper digestive tract and the bile are significantly changed during the process of acute liver injure in mice.

关 键 词：急性肝衰竭 肠道菌群 16S RDNA 生物多样性分析 LEfSe分析 

分 类 号：R575.3[医药卫生—消化系统]