社会隔离对BALB／c小鼠认知功能和海马5-HT2CR、ADAR1表达的影响  

Effects of social isolation on the cognition and expression of 5-HT2C receptor and ADAR1 in BALB/c mice

作　　者：徐红[1] 余伟志[3] 薛莹[3] 安冬[3] 陈薇[3] 梁金[3] 邢悦[3] 肖昭扬[4] 孙艺平[1] 唐一源[5] 殷盛明[2] 

机构地区：[1]大连医科大学基础医学院机能实验室,大连116044 [2]大连医科大学基础医学院生理学教研室,大连116044 [3]大连医科大学研究生,大连116044 [4]大连医科大学附属第二医院麻醉科,大连116027 [5]德克萨斯理工大学脑成像研究院,79409美国

出　　处：《中华行为医学与脑科学杂志》2017年第5期385-389,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（31201724,81471373）;辽宁省大学生科研创新活动立项项目（201310161008）

摘　　要：目的探讨社会隔离对BALB／c小鼠认知功能及5羟色胺2c受体（5-HT2Creceptor，5-HT2CR）和RNA腺苷脱氨酶1（adenosinedeaminasethatactOnRNA1，ADARl）表达的影响。方法选取健康BALB／c小鼠，按照随机原则（随机数字表法）分组，分别自生后21d起单笼饲养2周、4周和8周，建立社会隔离模型。对应的相同年龄正常群居饲养BALB／c小鼠作为相应对照组。采用新物体定位和新物体识别实验评估小鼠空间和非空间认知功能的改变，采用免疫蛋白印迹实验检测小鼠脑海马区5-HT2CR和ADARl表达的改变。结果新物体定位和新物体识别实验结果显示，与相应对照组相比，隔离2周组BALB／c小鼠的空间辨别指数明显降低[对照组为（0．075＋0．340），隔离组为（一0．653＋0．308），P〈O．05]，隔离2周和4周组BALB／c小鼠空间辨别指数无明显差异；隔离2周和4周组BALB／c小鼠非空间辨别指数明显降低[对照2周组为（0．088＋-0．210），隔离2周组为（-0．945＋-0．194），P〈0．05；对照4周组为（0．105＋0．267），隔离4周组为（-0．506＋-0．215），P〈0．05]，隔离8周组没有明显的改变；Westernblot结果显示，在海马区，与相应对照组相比，隔离2周BALB／c小鼠的5-HT2CR和ADARl蛋白表达明显减少[5-HT2CR：对照组为（1．025＋-0．144），隔离组为（0．891＋-0．026），P〈0．05][ADARl：对照组为（0．839--0．120），隔离组为（O．629＋-0．094），P〈0．05]。结论2周社会隔离可导致BALB／c小鼠的空间和非空间认知功能降低，同时引起BALB／c小鼠海马区5-HT2CR和ADARl蛋白表达的降低。Objective To explore the effects of social isolation on the cognition and expression of 5-HT2C receptor （5-HT2CR） and adenosine deaminase that act on RNA I （ ADAR1 ） in BALB/c mice. Methods The healthy BALB/c mice were isolated for 2,4, and 8 weeks individually since postnatal 21 days respectively to set up isolation mice model, the same age mice without isolation were regarded as control group. The new object location and the new object recognition tests were used to measure the spatial and non- spatial cognitive function, and western blot was used to measure the protein expression of 5-HT2CR andADAR1. Results The new object location test showed that the spatial discrimination index （DI） of BALB/c mice isolated for 2 weeks was decreased significantly compared with the control group（ control group was （0.075±0.340） ,isolation group was （ -0.653± 0.308 ） , P〈0.05） , and no obvious difference was found for the group isolated for 4 and 8 weeks. The new object recognition test showed that the non-spatial DI of BALB/c mice isolated for 2 and 4 weeks were decreased significantly compared with the control group（ control 2 weeks group was （0.088±0.210） ,isolation 2 weeks group was （-0.945±0.194） , P〈0.05; control 4 weeks group was （ 0.105 ± 0.267） , isolation 4 weeks group was （ - 0.506± 0.215 ） , P〈 0.05 ） , and no obvious difference was found for the group isolated for 8 weeks. Compared with the control group the expression of 5-HT2CR and ADAR1 in the hippocampus were decreased significantly for the group isolated for 2 weeks. （ 5-HT2CR ： control group was （ 1.025±0.144 ）, isolation group was （ 0. 891± 0.026）, P〈 0.05. ADAR1 ： con- trol group was （0.839±0.120） ,isolation group was （0.629±0.094） ,P〈0.05）. Conclusions Two week so- cial isolation results in the decrease of spatial and non-spatial cognitive function in BALB/c mice, in the meanwhile, social isolation stress results in the obvious decrease of 5-HT2C receptor and ADAR1 pro

关 键 词：社会隔离 认知功能 5羟色胺2c受体 RNA腺苷脱氨酶1 

分 类 号：R749[医药卫生—神经病学与精神病学]