采用非标记microRNA芯片筛选早产胎盘差异表达microRNA  

Identification of differentially expressed microRNA in preterm birth placentas by SHUT microarray

作　　者：孙健[1] 魏海霞[2] 汪云[1] 陈瑛[3] 

机构地区：[1]南京医科大学附属苏州医院产科,苏州215000 [2]山东省胜利石油管理局胜东社区锦苑卫生院,东营257000 [3]南京医科大学附属苏州医院新生儿科,苏州215000

出　　处：《中国优生与遗传杂志》2017年第5期8-10,7,共4页Chinese Journal of Birth Health & Heredity

基　　金：江苏省自然科学基金(BK2012600;BK20131151)

摘　　要：目的应用非标记microRNA芯片筛选在早产胎盘组织中差异表达的miRNA,并分析其相关的靶基因。方法收集早产儿16例为病例组,同期健康足月产儿18例为对照组,取胎盘组织。通过非标记miRNA芯片技术构建早产胎盘组织miRNA表达谱,采用荧光实时定量PCR进行验证,并分析差异表达miRNAs的靶基因。结果 miRNA芯片结果显示44种miRNAs在早产胎盘组织和对照组胎盘组织中差异表达,其中下调表达的11种,上调表达的33种,荧光实时定量PCR验证出miR-493-3p显著下调,mi R-4632-5p显著上调,与芯片结果一致。经软件分析,miRNA靶基因有IL16、SH2D2A、CCNG2、PHB、WNT1、CCDC92、GIT1和ST7L等相关靶基因。结论早产胎盘组织中存在差异表达的miRNAs,提示这些差异表达的miRNAs在早产的发生中有重要的调控作用。Objective： To explore the differentially expressed microRNA in human preterm placentas and predict their target genes. Methods： Placental tissue samples were collected soon after the placentas were delivered from 16 preterm birth patients, and 18 term birth women. Microarray of stacking hybridization-based universal tag assay was used to screen the differentially expressed miRNA and qRT-PCR method was used to confirm. The relevant target genes were predicted using the online database TargetScan, MicroTar, RNAhybrid, PicTar and Miranda. Results： 44 miRNAs were differentially expressed in preterm placentas compared to control group, among which 11 were down-regulated, and 33 were up-regulated. Down-regulation of miR-493- 3p and up-regulation of miR-4632-5p were confirmed by qRT-PCR results. The relevant target genes predicted were ILl6, SH2D2A, CCNG2, PHB, WNT1, CCDC92, GIT1 and ST7L etc. Conclusion： There is significant differential miRNA expression in preterm birth placentas, indicating that those miRNAs may play an important regulation role in preterm birth.

关 键 词：早产 微小核糖核酸 芯片 SHUT平台 

分 类 号：R440[医药卫生—诊断学] R714.21[医药卫生—临床医学]