双向固体发酵前后钩吻中生物碱含量变化研究  

A Study on Variation of Alkaloid Contents in Gelsemium Before and After Bidirectional Solid Fermentation

作　　者：李德森[1] 郑雅媗[2] 陈亮[3] 黄美霞[1] 谌赛男 吴水生[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建卫生职业技术学院,福建福州350101 [3]福建中医药大学附属福州中医院,福建福州350101

出　　处：《陕西中医药大学学报》2017年第3期63-68,共6页Journal of Shaanxi University of Chinese Medicine

基　　金：福建省中医药科研课题(wzzy201310);福州市科技计划项目(2014-S-140-1);福州市卫生系统科技计划项目(2013-S-wt9);国家大学生创新创业项目(201410393012)

摘　　要：目的研究钩吻被多种药用真菌固体发酵后生物碱含量的变化。方法运用双向固体发酵的原理与技术,将钩吻生品作为"药性基质",灵芝、云芝、茯苓等5种不同药用真菌分别接种到灭菌的钩吻"药性基质"上,在特定条件下进行发酵,得到钩吻"药性菌质"。采用HPLC法对发酵前后钩吻中钩吻碱甲、钩吻素子、钩吻素己、钩吻绿碱、胡蔓藤碱乙的含量进行测定。结果在同一发酵条件下,5种不同真菌对钩吻的发酵情况有所不同,"药性菌质"中各生物碱成分含量与钩吻生品相比,均呈不同程度下降的趋势,且色谱图中显示出一个新产生的未知成分。结论双向固体发酵后,菌丝体生长良好的钩吻发酵物中钩吻生物碱成分发生了质和量的明显变化,该法可能对钩吻的减毒增效物质基础研究具有重要意义。Objective： To determine the content of alkaloids in solid gelsemium fermented by medicinal fungus. Method ： With the principle and technique of bi - directional solid fermentation, the raw sterilized gelsemium, as a kind of drug matrix, was inoculated five different drugs as ganoderma lucidum, trametes versicolor, poria cocos etc. , and fer- mented in specific conditions to obtain the needed property of medicinal fungal substance. The HPLC method was used to detect the contents of gelsemine, koumine, gelsenicine, gelsevirine and humantenine before and after the experiment. Results ： Under the same circumstance, the five different fungus implementing different effects on fermentation of gelse- mium, the contents of alkaloids in inoculated gelsemium tended to decrease to varying degrees and a new - born un- known result showed in the chromatogram map after the comparison of those factors with the raw gelsemium. Discus-sion ： After bi - directional solid fermentation, the content of alkaloids in good - growth inoculated gclsemium changes a lot and the quality alters significantly, which may exert important function on the basic researches of the drug＇ s toxin re- lieving and curative effect strengthening.

关 键 词：钩吻 双向固体发酵 钩吻生物碱 HPLC 

分 类 号：R914[医药卫生—药物化学]