紫杉醇对实验性自身免疫性脑脊髓炎大鼠CD28、CTLA-4表达及BAFF的影响  

The effects of paclitaxel on the levels of CD28,CTLA-4 and BAFF in experimental autoimmune encephalomyelitis

作　　者：张淑江 李作孝 

机构地区：[1]西南医科大学附属医院神经内科,四川泸州646000

出　　处：《中国比较医学杂志》2017年第5期71-75,共5页Chinese Journal of Comparative Medicine

基　　金：泸州市科技创新苗子项目(2016-R-70 20/24);西南医科大学附属医院院级课题

摘　　要：目的探讨紫杉醇对实验性自身免疫性脑脊髓炎(experimental autoimmunencephalomyelitis,EAE)大鼠脑组织中CD28、CTLA-4表达及脾组织上清液中B细胞刺激因子(BAFF)含量及其意义。方法按随机数字法将50只Wistar大鼠分为5组:紫杉醇(PTX)小、中、大3个剂量组(PTX剂量分别为1 mg/kg,2 mg/kg,4 mg/kg)、正常对照组,EAE对照组,每组10只。用豚鼠脊髓制成GPSCH并与CFA等体积混合制成免疫抗原,注入大鼠双足侧足垫(2 m L/kg)进行EAE造模,自造模后,PTX各剂量组腹腔注射紫杉醇,连续10 d。正常对照组及EAE对照组均给予腹腔注射0.9%NaCl 2 m L。采用脑组织学评分评估实验大鼠脑组织炎症浸润情况,取大鼠脑组织采用流式细胞仪测定脑组织CD28、CTLA-4含量,取脾组织并制成上清液,采用ELISA测定脾组织中BAFF含量。结果 PTX各剂量组脑组织评分均低于EAE对照组,各组间差异有显著性(P<0.01);PTX各剂量组大鼠脑组织中CD28表达均低于EAE对照组,各组间差异有显著性(P<0.01);CTLA-4表达均高于EAE对照组,各组间差异有显著性(P<0.01);脾组织上清液中BAFF含量均低于EAE对照组,各组间差异有显著性(P<0.01)。结论 PTX可降低EAE大鼠神经功能障碍评分,其机制可能涉及调节大鼠脑组织中CD28、CTLA-4的表达及脾组织上清液中BAFF含量,从而发挥对EAE的防治作用。Objective To discuss the effects of Paclitaxel（PTX） on levels of CD28 and CTLA-4,B lymphocyte stimulator（BAFF） in experimental autoimmune encephalomyelitis（EAE）.Methods The 50 rats were divided into 5 groups by the random number table, 10 rats in each group,the doses of small group,Middle group, High group were 1 mg/kg,2 mg/kg,4 mg/kg by intraperitoneal injection for 10 consecutive days, the normal group and model group were injected 0.9% NS 2 mL,Using brain tissue score to estimate the neurological dysfunctions of rats.Using flow cytometry to detect the levels of CD28 and CTLA-4,using enzyme-linked immunosorbent （ELISA） to detect the levels of BAFF.Results The brain tissue score in PTX experimental groups were lower than model group,the comparative differences between groups were statistically significant（P 〈 0.01）;The levels of CD28 in PTX groups were lower than EAE group,the comparative differences between groups were statistically significant（P 〈 0.01）.The levels of CTLA-4 in PTX groups were higher than EAE group,the comparative differences between groups were statistically significant（P 〈 0.01）;the content of BAFF in all PTX groups were lower than EAE control group.Conclusions PTX could decrease the brain tissue score,the mechanism may adjust the express of CD28、CTLA-4 in brain and the expression of BAFF.PTX may have preventive and therapeutic effects on EAE rats.

关 键 词：紫杉醇 实验性自身免疫性脑脊髓炎 CD28 CTLA-4 B细胞刺激因子 

分 类 号：R-33[医药卫生]