血清学组合检测多种肿瘤相关抗原在食管鳞癌诊断中的意义  

Diagnostic Value of Combined Serological Examination of Multiple Tumor-Associated Antigens in Esophageal Squamous Cell Carcinoma

作　　者：刘博[1] 吕洋[2] 刘军超[1] 李坤[1] 李秀娟[2] 乔海霞[2] 

机构地区：[1]河北北方学院附属第一医院病理科,河北张家口075000 [2]河北北方学院组胚教研室,河北张家口075000

出　　处：《河北北方学院学报（自然科学版）》2017年第6期6-8,12,共4页Journal of Hebei North University:Natural Science Edition

基　　金：河北省科技厅计划项目(No.15277707D);河北省卫生厅科学技术指导项目(No.20140220)

摘　　要：目的探讨血清学组合检测多种肿瘤相关抗原(TAA)在食管鳞癌(ESCC)诊断中的意义。方法应用酶联免疫吸附法(ELISA)检测96例ESCC患者和63例健康对照者血清中4种TAA(CEA、Cyfra21-1、SCCA、NSE)的表达水平,分析组合检测这些血清TAA对ESCC诊断的特异性和灵敏性。结果 CEA、Cyfra21-1、SCCA、NSE在ESCC患者血清中的表达均高于健康对照组,差异有统计学意义(P<0.05)。单个检测ESCC患者血清中TAA阳性率普遍偏低,阳性率分别为10.4%(CEA)、17.7%(Cyfra21-1)、14.6%(SCCA)及25.0%(NSE)。对以上4种TAA进行逐步组合检测,发现血清学CEA、Cyfra21-1、SCCA、NSE联合诊断ESCC的敏感度可达44.8%,特异度能达72.4%。约登指数从1种TAA的0.066~0.309提高到4种TAA组合的0.124~0.435。结论血清学组合检测CEA、Cyfra21-1、SCCA、NSE对ESCC具有较高的诊断价值,对了解ESCC的发生、发展及转归有重要意义。Objective To evaluate the combined diagnostic value of multiple tumor-associated antigen （TAA） in esophageal squamous cell carcinoma （ESCC）. Methods Expressions of four TAAs including CEA, Cyfra21-1, SCCA and NSE in sera from 96 cases of ESCC and 63 cases of normal individuals were detected by ELISA. Results The expressions of CEA, Cyfra21-1, SCCA and NSE in ESCC were all sig- nificantly higher than those in normal individuals （P〈0.05）. The positive rates of tl？e four TAAs in ES- CC were generally low （10.4% （CEA）, 17.7%（Cyfra21-1）, 14.6% （SCCA） and 25.0% （NSE））. With the successive addition of TAA to a final total of four antigens, there was a stepwise increase of posi- tive reactions reaching to sensitivity of 44.8% and specificity of 72.4%. The Youdens index （YI） in- creased from 0. 066-0. 309 to 0. 124%. 435. Conclusion Multiple tumor markers combined detection may have higher diagnostic value for ESCC and be of great significance for genesis, development and out- come of ESCC.

关 键 词：食管鳞癌 肿瘤相关抗原 CEA CYFRA21-1 SCCA NSE 

分 类 号：R735.1[医药卫生—肿瘤]