MicroRNA-195对胶质瘤U251细胞株CCND1表达的影响  

Effect of microRNA-195 on the expression of CCND1 in glioma U251 cell

作　　者：徐勇明[1] 任书娴 常璐[1] 曲志钊 李亚娜[2] 王宏勤[2] 

机构地区：[1]山西医科大学研究生院,太原030001 [2]山西医科大学第一医院神经外科,太原030001

出　　处：《中华神经创伤外科电子杂志》2017年第3期155-158,共4页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：国家自然科学基金(81470115)

摘　　要：目的研究micro RNA-195对胶质瘤U251细胞株CCND1表达的影响。方法通过脂质体Lipofectamin 2000瞬时转染法将micro RNA-195过表达、micro RNA-195抑制表达转染胶质瘤细胞株U251。以空白转染组作为空白对照组(K组)、micro RNA-195过表达转染组为M组、micro RNA-195抑制表达转染组为I组。Real-time PCR检测转染前后micro RNA-195的含量以确保转染效率;再通过real-time PCR检测转染前后CCND1m RNA的表达变化;western blotting检测胶质瘤细胞U251中CCND1的表达水平。结果 Real-time PCR结果显示M组CCND1m RNA的表达低于I组(M:758.264<I:1398.04),western blotting实验显示M组相对I组CCND1表达减少(M:2029<I:22365.66)。结论 Micro RNA-195对胶质瘤U251细胞株CCND1表达有影响。Objective To research the effect of microRNA-195 on the expression of CCND1 in glioma U251 cell. Methods MicroRNA-195 mimics, microRNA-195 inhibitors were transfected into human glioma U251 cell by lipofectamine 2000 transient transfection. The microRNA-195 mimics transfection group was M group, and the microRNA-195 inhibitors transfection group was group I（blank control group, K group）. Real-time PCR was used to detect the content of microRNA-195 before and after transfection to ensure the transfection efficiency. The expression of CCND1 mRNA was detected by real-time PCR. The expression of CCND1 in glioma U251 cells was detected by western blotting. Results Real-time PCR results showed： the expression of CCND1 mRNA in group M was lower than that in group I（M：758.264〈I：1398.04）; western blotting： the expression of CCND1 was decreased in M group compared with I group （M：2029〈I：22365.66）. Conclusion MicroRNA-195 has an effect on the expression of glioma U251 cell line CCND1.

关 键 词：微小RNA-195 细胞周期蛋白D1 胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]