Stomatin样蛋白2基因调控Wnt／β-连环蛋白信号通路对胃癌细胞增殖凋亡的机制  

The mechanism of stomatin - like protein 2 gene regulating Wnt/β - catenin signaling pathway on proliferation and apoptosis of gastric cancer cells

作　　者：肖开提·阿不都哈德尔[1] 许新才[2] 迪力旦·纳斯尔[1] 刘光世[2] 张文斌[2] 

机构地区：[1]新疆医科大学第一附属医院干部病房外科,乌鲁木齐830054 [2]新疆医科大学第一附属医院胃肠肿瘤外科,乌鲁木齐830054

出　　处：《中华实验外科杂志》2017年第6期976-978,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨人Stomatin样蛋白2（SLP-2）基因调控Wnt／β-连环蛋白（β-catenin）信号通路对胃癌细胞增殖凋亡的影响及机制。方法Western blot检测SLP-2基因在人胃癌组织中的表达；将NC-小干扰RNA（siRNA）、SLP-2-siRNAl、SLP-2-siRNA2转染人胃癌SGC-7901细胞，未转染任何siRNA的作为对照组，Western blot检测转染后各组细胞中SLP-2蛋白表达；细胞转染48h后，流式细胞仪检测细胞凋亡；Western blot检测活化的的Cleaved含半胱氨酰的天冬氨酸特异性蛋白酶-3（Caspase-3）、B—catenin、c-Myc、细胞周期蛋白D1（CyclinD1）蛋白表达。结果胃癌组织中SLP-2的蛋白表达（0．283±0．016）显著高于癌旁组织（0．082±0．016）（t=7．789，P=0．003）；SLP-2-siRNA2组的抑制效果更明显，选择作为后续研究；LP-2-siRNA组（16．62±1．32）％细胞凋亡率及Cleaved Caspase-3蛋白表达（1．421±0．210）均显著高于对照组（1．56±0．56）％、（0．510±0．083），B—catenin（0．157±0．024）、c—Myc（0．039±0．016）、CyclinD1（0．052±0．012）蛋白表达显著低于对照组（0．311±0．036、0．137±0．026、0．183±0．021）（t凋亡率=9．324，P凋亡率=0．001；tβ-catenin=8．868，Pβ-catenin=0．003；tc-Myc=9．112，Pc-Myc=0．002；tcyclin D1=9．987，Pcvclin。D1=0．001）。结论SLP-2在胃癌中高表达，沉默SLP-2的表达能抑制人胃癌SGC-7901细胞增殖和促进细胞凋亡，其机制与Wnt/β—catenin信号通路的调控有关。Objective To investigate the effect of stomatin - like protein 2 （ SLP - 2） gene on the proliferation and apoptosis of gastric cancer cells by Wnt/β - eatenin signaling pathway. Methods The expression of SLP - 2 gene in human gastric cancer tissues was detected by Western blotting; NC - siRNA, SLP -2 - siRNA1 and SLP -2 - siRNA2 were transfected into human gastric cancer SGC -7901 cells, and not transfected with any siRNA as the control group ; the expression of SLP - 2 protein after transfected cells was detected by Western blotting; cells transfected for 48 h, cell apoptosis was detected by flow cytometry; Cleaved Caspase- 3, β -catenin, c -Myc, Cyclin Dlprotein expression was detected by Western blotting. Results The expression of SLP- 2 protein in gastric carcinoma （0. 283 ± 0. 016 ）was significantly higher than paracancerous tissues （ 0. 082 ± 0. 016 ）, （ t = 7. 789, P = 0. 003 ） ; the inhibitory effect of SLP-2- siRNA2 group was more obvious, and was choiced as a follow -up study; the apoptosis rate （16. 62 ± 1.32）% of cells and Cleaved Caspase -3 （1. 421 ± 0. 210） protein expression in SLP- 2 -siRNA group were significantly higher than the control group （1.56 ± 0. 56）%, （0. 510 ± 0. 083 ）, β -catenin （0. 157 ± 0. 024 ）, （0. 039 ± 0. 016 ）, Cyclin D1 （ 0. 052 ± 0. 012 ） protein expression was significantly lower than the control group （ 0. 311 ± 0. 036, 0. 137 ± 0. 026, 0. 183 _± 0. 021 ） （ tapoptosis rate = 9. 324,Papoptosis rate = 0. 001 ; tCleaved Caspase-3= 6. 112, PCleaved Caspase -3 = 0. 006 ; tβ -catenin = 8. 868, Pβ-catenin= 0. 003 ; tc-Myc =9. 112, Pc-Myc =0.002; tCydinD1 =9.987, PcydlnD1 =0.001）. Conclusion SLP -2 is highly ex- pressed in gastric cancer, Silencing SLP -2 expression can inhibit the proliferation and apoptosis of human gastric cancer cell line SGC - 7901, and its mechanism is related to the regulation of Writ/β - eatenin sig- naling pathway.

关 键 词：Stomatin样蛋白2 Wnt/β-连环蛋白信号通路 胃癌 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]