辣椒疫霉侵染拟南芥的酵母双杂交cDNA文库构建及其应用  

Construction and application of a yeast two-hybrid cDNA library from Arabidopsis thaliana infected by Phytophthora capsici

作　　者：汪俭[1,2] 李敏[2,3] 张鹏[2,4] 纪志远[2] 马立安[1] 乔永利[2] 

机构地区：[1]长江大学生命科学学院,荆州434025 [2]中国农业科学院作物科学研究所,北京100081 [3]长江大学园艺园林学院,荆州434025 [4]长江大学农学院,荆州434025

出　　处：《植物病理学报》2017年第3期416-421,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31522045;31571696);中国农业科学院科技创新工程项目资助

摘　　要：辣椒疫霉（Phytophthora capsici）是一种重要的植物病原卵菌,其寄主范围较广,可引起辣椒、番茄、黄瓜、南瓜等多种重要蔬菜作物的疫病。辣椒疫病是一种毁灭性病害,在田间发病经常造成幼苗猝倒、茎秆枯死和果实腐烂,危害相当严重,每年给全世界蔬菜产业造成的损失就高达10亿美元。辣椒疫病1918年在美国首次发生,之后在世界范围内迅速扩展。Phytophthora capsici is an oomycete plant pathogen that causes blight and fruit rot of peppers and other important vegetable crops, and results in multi-billion dollar losses in crop production annually. In this study, we employed A. thaliana - P. capsici pathosystem to investigate the pathogenic mechanism of oomycete pathogens. Total RNA was isolated from stems, flowers and infected leaves at 6 and 12 h after inoculation with P. capsici strain LT263. An Arabidopsis thaliana cDNA library was constructed at an early stage of P. capsici in- fection. The unamplified cDNA library was consisted of 4.0×10^6 independent colonies, and the length of inserted fragments was ranged from 0.5 kb to 3.0 kb with an average size of 1.5 kb. The cDNA library was then applied to screen for PINP1 interacting proteins, and 157 putative nuclear proteins were obtained by analyzing protein sequence and subcellular localization. Functional analysis in term of Gene Ontology showed that these candidate proteins were involved in 7 biological processes and 6 molecular functions. These data suggest that this is a high quality library and suitable for identifying the interacting proteins of an interested protein in the A. thaliana and P. capsici pathosystem.

关 键 词：辣椒疫霉 酵母双杂交 文库构建 cDNA 拟南芥 应用 侵染 辣椒疫病 

分 类 号：S432.44[农业科学—植物病理学]