猪细小病毒病毒样颗粒的体外组装  

Self-assembly of virus-like particles of porcine parvovirus

作　　者：宋品[1,2] 孙世琪[2] 董虎[2] 滕志东[2] 曹随忠[1] 郭慧琛[2] 余树民[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川成都611130 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《中国兽医科学》2017年第6期707-712,共6页Chinese Veterinary Science

基　　金：国家"十二五"科技支撑计划项目(2013BAD12B00);国家国际科技合作专项(2014DFA31890);国家自然科学基金项目(31672592)

摘　　要：为了研制猪细小病毒(PPV)病毒样颗粒(VLPs)疫苗,将合成的PPV VP2全长基因克隆到带有His标签和小分子泛素相关修饰物(SUMO)标签的p SMK表达载体中,在大肠杆菌中表达可溶性PPV VP2蛋白。对表达的重组融合蛋白,用特异性蛋白酶切除其SUMO标签,结果显示PPV VP2蛋白可自我组装成VLPs。进一步研究发现,VLPs的组装效率与pH值、离子强度关系密切。本研究通过大肠杆菌表达系统获得的具有良好反应原性的PPV VLPs,为研制PPV VLPs疫苗奠定了良好的基础。In order to develop VLPs vaccines against porcine parvovirus,the PPVVP2 gene was cloned into a pSMKexpression vector containing a His tag and small ubiquitin-like modifier（SUM0） tag that can promote the soluble expression of PPVVP2 proteins in Escherichia coli cells. After removal of the SUM0 tag from the expressed recombinant fusion protein,the recombinant PPVVP2 protein had self-assembled into VLPs. Further study showed that the self-assembly efficiency of VLPs was affected by different pH levels and ionic strengths. In conclusion,PPV VLPs with highly reactionogenicity were obtained by the prokaryote expression system,which may pave a new way for research and development of a VLPs vaccine against PPV.

关 键 词：猪细小病毒 病毒样颗粒 原核表达 VP2基因 

分 类 号：S852.651[农业科学—基础兽医学]