Structure and Function Analysis of Canine ZP3 Protein  

作　　者：Zhao Qintao Zhang Jianhua Ye Junhua 

机构地区：[1]Nanchang Police Canine Base of the Ministry of Public Security,Nanehang 330100,China

出　　处：《Animal Husbandry and Feed Science》2017年第2期74-79,共6页动物与饲料科学（英文版）

基　　金：Supported by National Key Research and Development Plan in the 13th Five Year(2016YFD0501008)

摘　　要：To further study the biological function and mechanism of ZP3 in domestic canine （ CaMs lupu familiaris） , the coding sequence （CDS） of ZP3 gene was searched from NCBI database using bioinformatics method, and further transformed into protein sequence via MAGE software. The primary, secondary and terti- ary structure of protein was predicted with ExPASy, BLAST and DNA Star bioinformatics online software and program; the evolution and selected sites of ZP3 pro- tein extracted from 11 species were analyzed using PAML software; the conservation of ZP3 protein gene was analyzed with Predictprotein and Weblogo program; the tertiary structure of protein was edited by Python and PyMOL. The results showed that canine ZP3 gene encoded 426 amino acids, and the encoding product was a hydrophilic transmembrane protein with signal peptide; the 1 -23ra amino acids were signal peptide areas, and transmembrane domain distributed in the （x-helix area of the 386th -408th amino acids; four loci were affected by phosphorylation, and these phosphorylation sites might be associated with signal transduction ; there were nine protein binding sites on ZP domain; a high variation region was found in 325 -385 section of ZP3, and most of phosphorylation and selected amino acid sites were distributed in this area. This indicated that the area had experienced rapid evolution, suggesting that ZP domain and the high variation area might be in- volved in interaction of sperm and egg.To further study the biological function and mechanism of ZP3 in domestic canine （ CaMs lupu familiaris） , the coding sequence （CDS） of ZP3 gene was searched from NCBI database using bioinformatics method, and further transformed into protein sequence via MAGE software. The primary, secondary and terti- ary structure of protein was predicted with ExPASy, BLAST and DNA Star bioinformatics online software and program; the evolution and selected sites of ZP3 pro- tein extracted from 11 species were analyzed using PAML software; the conservation of ZP3 protein gene was analyzed with Predictprotein and Weblogo program; the tertiary structure of protein was edited by Python and PyMOL. The results showed that canine ZP3 gene encoded 426 amino acids, and the encoding product was a hydrophilic transmembrane protein with signal peptide; the 1 -23ra amino acids were signal peptide areas, and transmembrane domain distributed in the （x-helix area of the 386th -408th amino acids; four loci were affected by phosphorylation, and these phosphorylation sites might be associated with signal transduction ; there were nine protein binding sites on ZP domain; a high variation region was found in 325 -385 section of ZP3, and most of phosphorylation and selected amino acid sites were distributed in this area. This indicated that the area had experienced rapid evolution, suggesting that ZP domain and the high variation area might be in- volved in interaction of sperm and egg.

关 键 词：CANINE ZP3 BIOINFORMATICS Structure prediction Biological function 

分 类 号：S829.2[农业科学—畜牧学]