肝核受体激动剂对人表皮鳞状细胞癌SCL-1细胞增殖作用的影响  

Effects of LXRs agonists on the proliferation of SCL-1 cells

作　　者：李漪倩 诸蓉 蔡小薇[1] 张斌[1] 王儒鹏[1] 何威[1] 

机构地区：[1]第三军医大学附属新桥医院,重庆400037

出　　处：《实用皮肤病学杂志》2017年第2期65-69,共5页Journal of Practical Dermatology

基　　金：国家自然科学基金青年基金资助项目(81502369)

摘　　要：目的探讨肝核受体LXRs激动剂T0901317人表皮鳞状细胞癌SCL-1细胞增殖的影响及可能机制。方法 CCK-8法检测不同浓度T0901317(1.0、10.0、20.0μmol/L)作用SCL-1细胞24、48、72 h后细胞增殖率的变化。流式细胞仪检测不同浓度肝核受体激动剂对SCL-1细胞周期分布的影响。反转录-聚合酶链反应(RT-PCR)及免疫印迹试验(Western blot)检测肝核受体激动剂对细胞周期相关因子PCNA、p21、p27、CCND1表达水平的影响。结果 T0901317可明显抑制SCL-1细胞增殖,并呈现一定的时间和浓度依赖性;肝核受体激动剂作用于细胞株后,SCL-1细胞的G1期百分率上升,而S期、G2期百分率下降;且在一定浓度范围内导致细胞周期因子p21的m RNA及蛋白表达增加,而其他细胞周期相关因子表达未见明显变化。结论肝核受体LXRs激动剂可能通过促进细胞周期调节负性因子p21的表达,导致细胞周期静息甚至停滞于G1期,从而抑制细胞增殖。Objective To detect the effects of LXRs agonists T0901317 on human cutaneous squamous cell carcinoma SCL-1. Methods 24,48 and 72 hours after T0901317（1.0, 10.0, 20.0 μmol/L） with different concentrations acting on SCL-1 cells, CCK-8kit assay was performed to detect cell proliferation. After having been treated with T0901317（1.0, 10.0, 20.0 μmol/L） for 24 hours, SCL-1 cells cycle progression was analyzed by flow cytometry. RT-PCR and Western blot were used to detect the expression of cell cycle related factors＇ genes and proteins. Results T0901317 inhibited the proliferation of SCL-1 cells in the concentration and time dependant manners. The proportion of SCL-1 cells in Gl-phase increased, while that in G2- and S-phase decreased after having been treated with T0901317 （1.0, 10.0,20.0 μmol/L） for 24 hours. The result of PCR and Western blot showed that the expression of p21 protein was up-regulated, but the other cell cycle factors had no obviously changes. Conclusion The inhibition of SCL-1 cells proliferation by LXRs agonists is involved in Gl-phase arrest, which might be related to the increased expression of p21 factor.

关 键 词：癌 鳞状细胞 SCL-1细胞 LXRs P21 G1期阻滞 

分 类 号：R739.5[医药卫生—肿瘤]