MMP-2、TIMP-2在食管鳞状细胞癌的表达与临床意义  

Expression of MMP-2 and TIMP-2 in esophageal squamous cell carcinoma and its clinical significance correlations

作　　者：袁继宝 庞瑶[1,2,4] 熊海科 朱自江[1,2,4] 薛继军[3] 朱小康[3] 赵海龙 郑凤长[3] YUAN Ji-bao PANG Yao XIONG nai-ke ZHU Zi-jiang XUE Ji-jun ZHU Xiao-kang ZHAO Hai-long ZHENG Feng-zhang(The Clinical School, Gansu College of Traditional Chinese Medicine ,Lanzhou Gansu 730000 Department of Thoracic Surgery, The Tumor Hospital of Gansu,Lanzhou Gansu 730050 Department of Thoracic Surgery, The People＇s Hospital of C, ansu,Lanzhou Gansu 730000,China)

机构地区：[1]甘肃中医学院临床医学院,甘肃兰州730000 [2]泰山医学院附属成武医院胸外科 [3]甘肃省肿瘤医院胸外科,甘肃兰州730050 [4]甘肃省人民医院胸外科,甘肃兰州730000

出　　处：《蚌埠医学院学报》2017年第3期297-301,共5页Journal of Bengbu Medical College

基　　金：国家自然科学基金项目(81060205);甘肃省杰出青年基金项目(1308RJDA009)

摘　　要：目的:探讨基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制剂-2(TIMP-2)在食管鳞状细胞癌(ESCC)中的表达与术后复发或转移和预后之间的关系。方法:采用组织芯片技术,用免疫组织化学法检测ESCC术后存活5年以上且无复发转移组(A组)和术后在1年内发生复发转移死亡组(B组)肿瘤组织细胞中的MMP-2、TIMP-2表达情况。结果:MMP-2、TIMP-2在正常食管鳞状上皮中不表达或少量表达,在ESCC组织中有不同程度的表达,但MMP-2和TIMP-2在B组的表达水平均显著高于A组(P<0.01)。MMP-2表达与肿瘤分化程度和有无淋巴结转移均有相关关系(P<0.05和P<0.01);TIMP-2表达与分化程度和有无淋巴结转移率亦均有相关关系(P<0.01和P<0.05)。TIMP-2表达与MMP2表达呈正相关关系(P<0.01)。结论:MMP-2、TIMP-2表达在食管癌组织中高表达,其表达情况与食管癌术后发生复发转移有明显相关性,联合检测MMP-2、TIMP-2表达对预测ESCC术后发生复发转移和预后有重要价值。Objective： To investigate the expressions of the matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase inhibitor-2（TIMP-2） in esophageal squamous cell carcinoma（ESCC） ,and its correlations with the recurrence,metastasis and prognosis of tumor. Methods ： The expressions of MMP-2 and TIMP-2 in tumor tissue of the postoperative patients with ESCC were detected using the immunohistochemistry with Tissue Chip technique. The patients without recurrence and metastasis survived for over 5 years （ group A） ,the patients with recurrence and metastasis died within 1 year （group B）. Results： The squamous epithelium of the normal esophagus did not express or expressed little MMP-2 and TIMP-2. The expressions of MMP-2 and TIMP-2 in ESCC tissue were identified, but the expression levels of MMP-2 and TIMP-2 in group B were significantly higher than those in group A（ P 〈 0.01 ）. The expression levels of MMP-2 and TIMP-2 were correlated with the tumor differentiation and lymph node metastasis （P 〈 0.05 and P 〈 0.01 ）. The expression level of TIMP-2 was positively correlated with the MMP-2 （ P 〈 0.01 ）. Conclusions ： MMP-2 and TIMP-2 are overexpressed in esophageal carcinoma tissue, and which are significantly correlated with the recurrence or metastasis of tumor after esophagectomy. The combined detection of MMP-2 and TIMP-2 has important value in predicting the recurrence or metastasis of tumor after operation of ESCC.

关 键 词：食管肿瘤 基质金属蛋白酶-2 基质金属蛋白酶抑制剂-2 复发 转移 

分 类 号：R735.1[医药卫生—肿瘤]