抗炎抗凝双效融合蛋白TAP-SSL5对大鼠长期毒性研究  

The long-term toxicity study of TAP-SSL5 fusion protein

作　　者：房兆飞[1,2] 胡厚源[1] FANG Zhao-fei HU Hou-yuan(Department of Cardiology, Southwest Hospital, Third Military Medical University, Chongqing 400038 Department of Cardiology, Zhoushan Hospital of Zhejiang,Zhoushan 316000, China)

机构地区：[1]第三军医大学西南医院心内科,重庆400038 [2]浙江省舟山医院心内科,浙江舟山316000

出　　处：《中国生化药物杂志》2017年第5期7-9,13,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家高技术研究发展计划(863计划,2009AA02Z115);国家重大新药创制课题(2013ZX09103003-001)

摘　　要：目的研究抗炎抗凝双效融合蛋白TAP-SSL5对大鼠的长期毒性,评价其用药安全性。方法 SD大鼠隔天腹腔注射给药TAP-SSL5 1 mg/kg一次,连续给药8周,恢复1周,检测大鼠一般行为状态、体质量变化、血液常规及血液生化、脏器系数等指标。结果各组大鼠一般行为状态正常、体征正常、体质量无明显异常;血液常规和生化指标未见异常变化;各组动物器官组织病理检查未见明显异常;各组间脏器重量及脏器系数未见明显差异。结论融合蛋白TAP-SSL5对大鼠长期毒性小,提示其用药安全性较高。Objective To investigate the long-term toxicity of anti-inflammatory and anticoagulant tick anticoagulant peptide （TAP）-staphylococcal superantigen like protein-5 （SSL5） fusion protein in normal rats.MethodsSD rats were intraperitoneally injected with TAP-SSL5 （1mg/kg, every other day） for eight weeks and followed up for one week.The general behavior, weight, blood routine test, blood biochemistry and organ indexe were measured.ResultsOur results showed that there were no significantly difference between the TAP-SSL5 treated rats and the control on general behavior, weight, blood routine test, blood biochemistry, organ indexe and pathology.ConclusionThe fusion protein TAP-SSL5 with little long-term toxicity for rats is proved to be a safe drug.

关 键 词：金黄色葡萄球菌超抗原样蛋白-5 蜱抗凝血肽 融合蛋白 长期毒性 

分 类 号：R363[医药卫生—病理学]