柞蚕蛹虫草超微粉对小鼠免疫功能的增强作用  

Enhancement effect of Superfine Cordyceps militaris Powder on immune functions of mice

作　　者：宋佳[1] 聂铭喜 赵咏梅[3] 苗婷婷[1] 金梦迪[1] 李航[1] 李依寰 聂晶石 王春艳[1] SONG Jial NIE Mingxi ZHAO Yongmei MIAO Tingting JIN Mengdi LI Hang LI Yihuan NIE Jingshi WANG Chunyan(Department of Health Laboratory, School of Public Health, Jilin University, Changchun 130021, China Dunhua Silkworm Science and Technology Development Co. Ltd, Jilin Province, Dunhua 133700, China Department of Drug Administration, China-Japan Union Hospital, Jilin University, Changehun 130033, China)

机构地区：[1]吉林大学公共卫生学院卫生检验学教研室,吉林长春130021 [2]吉林省敦化市蚕业科技发展有限责任公司,吉林敦化133700 [3]吉林大学中日联谊医院药学部,吉林长春130033

出　　处：《吉林大学学报（医学版）》2017年第3期496-501,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目国际合作项目资助课题(20130413059GH);吉林省科技厅科技发展计划项目资助课题(20110940)

摘　　要：目的:探讨东北柞蚕蛹虫草超微粉对小鼠免疫功能的影响,为提高柞蚕蛹虫草的利用率提供依据。方法:40只小鼠随机分为阴性对照组,低(0.166 5g·kg^(-1))、中(0.333 3g·kg^(-1))和高剂量柞蚕蛹虫草超微粉组(0.999 9g·kg^(-1));每组10只。低、中和高剂量柞蚕蛹虫草超微粉组小鼠经口给予柞蚕蛹虫草超微粉30d,阴性对照组小鼠经口给予等量蒸馏水。测定各组小鼠体质量、脾脏指数和胸腺指数,采用小鼠淋巴细胞转化实验观察淋巴细胞转化能力,足跖增厚法检测小鼠迟发型变态反应水平,检测小鼠抗体生成细胞数、小鼠巨噬细胞吞噬鸡红细胞的吞噬率和吞噬指数。结果:与阴性对照组比较,各剂量柞蚕蛹虫草超微粉组小鼠体质量、脾脏指数和胸腺指数差异无统计学意义(P>0.05);中和高剂量柞蚕蛹虫草超微粉组小鼠淋巴细胞转化能力高于阴性对照组(P<0.05);中和高剂量柞蚕蛹虫草超微粉组小鼠迟发型变态反应水平高于阴性对照组和低剂量柞蚕蛹虫草超微粉组(P<0.05);各剂量柞蚕蛹虫草超微粉组小鼠抗体生成细胞数高于阴性对照组(P<0.05);各剂量柞蚕蛹虫草超微粉组小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬率和吞噬指数高于阴性对照组(P<0.05)。结论:东北柞蚕蛹虫草超微粉对小鼠免疫功能有增强作用,推荐剂量为0.333 3和0.999 9g·kg^(-1)。Objective： To investigate the effect of Superfine Cordyceps militarisv Powder on the immune functions of the mice, and to provide basis for improving the utilizable ratio of Cordyceps rnilitaris. Methods： A total of 40 mice were randomly divided into negative control group, low dose （0. 166 5 g · kg^-1 ）, middle dose（0. 333 3 g · kg^-1 ）, and high dose （0. 999 9 g ·kg^-1 ） of Superfine Cordyceps militaris Powder groups, and there were 10 mice in each group. The mice in different doses of Superfine Cordyceps militaris Powder groups were administered with the Superfine Cordyceps militaris Powder for 30 d by intragastric infusion respectively, whereas, the mice in negative control group were administered with the same volume of water for 30 d by intragastric infusion. The body weights, the spleen indexes and thymus indexes of the mice in various groups were measured; the lymphocyte transformation abilities of the mice in various groups were observed by lymphocyte transformation experiment ; the levels of delayed-type hypersensitivity reaction of the mice were examined with toe incrassation; the number of antibody forming cells, phagocytic rate and phagocytic index of chicken erythrocytes phagocytized by peritoneal macrophages of the mice were detected. Results： Compared with negative control group, the body weights, the spleen indexes and thymus indexes of the mice in different doses of Superfine Cordyceps militaris Powder groups had no significant differences （P〉0.05）. The lymphocyte transformation abilities of the mice in middle and high doses of Superfine Cordyceps rnilitarisPowder groups were higher than that in negative control group （P〈0.05）. The levels of delayed-type hypersensitivity reaction of the mice in middle and high doses of Superfine Cordyceps militaris Powder groups were higher than those in low dose of Superfine Cordyceps militaris powder group and negative control group （P〈0.05）. The numbers of antibody forming cells of the mice in low, middle and high doses

关 键 词：柞蚕蛹虫草超微粉 免疫力 吞噬指数 吞噬率 

分 类 号：R364.7[医药卫生—病理学]