复方五味子提取物的抗疲劳作用及其机制  

Anti-fatigue effect of compound schisandra extracts in mice and its mechanism

作　　者：王维[1] 刘聪[1] 蒋岩[1] 王松萍[1] 于慧[1] 敬舒[2] 庄文越[1] 王春梅[1] 陈建光[1] 李贺[1] WANG Wei LIU Cong JIANG Yan WANG Songping YU Hui JING Shu ZHUANG Wenyue WANG Chunmei CHEN Jianguangl LI He(Department of Pharmacology, College of Pharmacy, Beihua University, Jilin 132013, China Department of General Surgery, Affiliated Hospital, Beihua University, Jilin 130021, China)

机构地区：[1]北华大学药学院药理教研室,吉林吉林132013 [2]北华大学附属医院普通外科,吉林吉林132001

出　　处：《吉林大学学报（医学版）》2017年第3期502-506,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅重点科技攻关计划项目资助课题(20140204012);吉林省科技厅科技发展计划项目资助课题(20150312041ZG);吉林省吉林市科技局科研项目资助课题(20166018)

摘　　要：目的:探讨五味子、黄芪、刺五加和红景天混合提取物(CSE)对小鼠力竭游泳时间及其体内的血尿素氮(BUN)、血清乳酸(LD)、肝糖原、肌糖原水平、超氧化物歧化酶(SOD)活性及丙二醛(MDA)水平的影响,阐明其抗疲劳作用及其机制。方法:80只雄性ICR小鼠随机分为空白对照组、50mg·kg^(-1) CSE组、100mg·g-1 CSE组和200mg·kg^(-1) CSE组,每组20只。给药30d后,每组随机选取10只小鼠进行力竭游泳实验,记录小鼠力竭游泳时间。剩余10只小鼠游泳90min后处死,取血及组织标本,检测其体内BUN、LD、肝糖原、肌糖原、MDA水平及SOD活性;采用亚油酸-硫氰酸铁法测定CSE体外总氧化抑制率。结果:与空白对照组比较,50、100和200mg·kg^(-1) CSE组小鼠力竭游泳时间均明显延长(P<0.01);与空白对照组比较,100和200mg·kg^(-1) CSE组小鼠BUN和LD水平明显降低(P<0.05或P<0.01),肝糖原和肌糖原水平升高(P<0.05或P<0.01),SOD活性升高(P<0.05或P<0.01),MDA水平降低(P<0.05或P<0.01)。与100mg·kg^(-1) CSE组比较,200mg·kg^(-1) CSE组小鼠血清BUN和LD水平降低(P<0.01),肝糖原和肌糖元水平升高(P<0.05)。5g·L^(-1) CSE的总氧化抑制率可达76.94%。结论:CSE具有抗疲劳作用,其作用机制可能与抗氧化作用有关联。Objective： To study the effects of compound schisandra extracts （CSE） （schisandra, astragalus, acanthopanax, and rhodiola） on the exhaustive swimming time and the levels of blood urea nitrogen （BUN）, serum lactic acid （LD）, liver glycogen and muscle glycogene, superoxide dismutase （SOD） activity, and malonaldehyde （MDA） level in the mice and to charify its anti-fatigue effect and the mechanism. Methods： Eighty male ICR mice were randomly divided into blank control group, 50 mg · kg^-1 CSE group, 100 mg· kg^-1 CSE group, and 200 mg · kg^-1CSE group; there were 20 mice in each group. The mice were administered orally for 30 d. Then 10 mice were randomly selected for exhaustive swimming test in each group and the exhaustive swimming time of the mice was recorded. The remaining 10 mice in each group were used for 90 min swimming, then all the mice were sacrificed and the blood and tissue samples were taken for the measurement of the levels of BUN, LD, liver glycogen and muscle glycogen, the SOD activity and MDA level; the total inhibitory rate of oxidation of CSE in vitro was determined by linoleic acid-ferric thiocyanate method. Results.. Compared with blank control group, the exhaustive swimming time of the mice in 50, 100, and 200 mg · kg^-1 CSE groups were significantly increased （P〈0. 01） ; the levels of BUN and LD of the mice in 100 and 200 mg· kg^-1 CSE groups were significantly decreased （P〈0.05 or P〈0.01） ; the levels of liver glycogen and muscle glycogen of the mice in 100 and 200 mg·kg^-1 groups were significantly increased （P〈0.05 or P〈0.01）; whereas the SOD activities were significantly increased and the levels of MDA were decreased （P 〈0.05 or P〈0.01）. Compared with 100 mg· kg^-1 CSE group, the levels of serum BUN and LD of the mice in 200 mg·kg^-1CSE group were decreased （P〈0. 01）, and the levels of liver glycogen and muscle glycogen were increased （P〈O. 05）. The total inhibitory rate of oxidation of 5 g·L^-1 CSE was 76.94%.

关 键 词：五味子 抗疲劳 抗氧化 乳酸 小鼠 ICR 

分 类 号：Q58[生物学—生物化学] R332[医药卫生—人体生理学]