Taxol对宫颈癌U14细胞增殖、凋亡及α2,6-SA和ST6Gal mRNA表达的影响  

Effects of Taxol on proliferation,apoptosis,and mRNA expression of α2,6-sialic acid and ST6Gal in cervical carcinoma cell line U14

作　　者：高雪松[1] 王晓玉[1] 

机构地区：[1]暨南大学第一附属医院妇产科,广东广州510632

出　　处：《中国病理生理杂志》2017年第6期1038-1042,共5页Chinese Journal of Pathophysiology

基　　金：广东省药学会妇科肿瘤用药研究基金资助项目(No.2012D15);暨南大学第一临床医学院科研培育专项基金[2012]7号青年项目(No.9)

摘　　要：目的:研究泰素(Taxol)对小鼠宫颈癌U14细胞株增殖、凋亡以及α2,6-唾液酸(SA)和α2,6-唾液酸转移酶(ST6Gal)mRNA表达的影响,为进一步探讨宫颈癌Taxol化疗机制提供新思路。方法:Taxol处理U14细胞后,MTT检测Taxol对U14细胞的IC_(50)值。流式细胞术检测细胞α2,6-SA、细胞凋亡相关因子(Bcl-2、Bax、caspase8和caspase 3)、凋亡率和细胞周期改变。qPCR检测ST6Gal1和ST6Gal2 mRNA的表达。结果:与对照组相比,Taxol对U14细胞有明显的抑制作用,减弱α2,6-SA荧光强度,上调Bax表达,下调Bcl-2表达,降低Bcl-2/Bax比值,并增强caspase 8和caspase 3活性;Taxol处理显著增加U14细胞凋亡率及S期细胞和G2/M期细胞比率,此外还下调ST6Gal1 mRNA表达。结论:α2,6-SA和ST6Gal可能参与了Taxol对U14细胞细胞周期和凋亡调控的多重作用。AIM ： To study the effect of Taxol on the proliferation, apoptosis, and mRNA expressions of α2 ,6- sialic acid （SA） and α2,6-sialyltransferase （ST6Gal） in mouse cervical cancer cell line U14. METHODS： After the U14 cells were treated with Taxol, the IC50 value of Taxol to U14 cells was detected by MTT assay. The expression of α2,6-SA and apoptosis-related factors （ Bel-2, Bax, caspase 8 and caspase 3 ） , the apoptosis rate and cell cycle were determined by flow cytometry. The mRNA expression of ST6Gall and ST6Gal2 was detected by qPCR. RESULTS： As compared with control group, Taxol induced obvious U14 cell growth inhibition, reduced α2,6-SA expression, up-regulated Bax, down- regulated Bel-2, decreased the ratio of Bcl-2/Bax, enhanced caspase 8 and caspase 3 activity, increased the apoptotic rate and cell proportions of Sub-G1 and S phases,and induced G2/M phase arrest. Taxol also down-regulated the mRNA expres-sion of ST6Gal l , and slightly up-regulated the mRNA expression of ST6Gal2. CONCLUSION： α2,6-SA and ST6Gal are involved in the multiple effects of Taxol on modulation of the cell cycle and apoptosis in U14 cells.

关 键 词：泰素 细胞凋亡 细胞周期 α2 6-唾液酸 α2 6-唾液酸转移酶 

分 类 号：R730.23[医药卫生—肿瘤] R711.74[医药卫生—临床医学]