ELMO1在肝细胞癌中的表达及对肝癌细胞株迁移能力的影响  

Expression of ELMO1 in Hepatocellular Carcinoma and its Effects on Cell Migration

作　　者：黄文瑾[1] 席红利[1] 林晓丹[1] 余宏伟[1] 周同冲[1] 

机构地区：[1]广州医科大学附属肿瘤医院,广东广州510095

出　　处：《现代医院》2017年第5期718-721,共4页Modern Hospitals

基　　金：广州市医药卫生科技项目(编号:20161A010091)

摘　　要：目的探讨ELMO1蛋白在肝细胞癌中的表达情况及其对肝癌细胞株迁移能力的影响。方法利用Western blot技术检测ELMO1蛋白在肝癌组织和不同肝癌细胞株中的表达。构建过表达ELMO1的质粒及合成靶向抑制ELMO1表达的siRNA,分别转染至人肝癌细胞株SK-Hep-1中,利用Western blot技术检测ELMO1蛋白的表达及细胞Rac1的活性,用Transwell法检测细胞的侵袭能力。结果 ELMO1在正常肝细胞株LO_2和转移潜能最弱的HepG2中表达最低,在转移潜能最强的SK-HEP-1中表达最高。ELMO1在肝癌组织中的表达均高于癌旁组织。与转染空载体的SK-Hep-1细胞相比,转染ELMO1表达质粒的SK-Hep-1细胞中ELMO1的表达明显上调,细胞Rac1活性明显增强,Transwell穿过膜的细胞明显增多(P<0.05)。与转染阴性对照siRNA的SK-Hep-1细胞相比,转染ELMO1 siRNA的细胞中ELMO1的表达明显下调,细胞Rac1活性明显减弱,Transwell穿过膜的细胞数明显减少(P<0.05)。结论 ELMO1在肝细胞癌中的表达明显升高,促进细胞的迁移。Objective To investigate the expression of engulfment and cell motility protein 1 （ELMO1） in hepato-cellular carcinoma（ HCC） and its effects on cell migration. Methods The expression of ELMO1 in HCC cell lines and HCC tissues was assessed by Western blot. We used transient transfection with an ELMO1 expressing vector to over - express EL-MO1 protein in SK - HEP - 1 cells. Over - expression of ELMO1 was confirmed by Western blot. siRNAs specific to ELMO1 （ELMO1 siRNA） were used to knockdown ELMO1 expression. Rho Family Small GTPase Activation Assay, Western blots, transwell assay were used to determine the migration potential of cells. Students test was employed for statistical analysis. Re-sults The expression of ELMO1 protein was obviously up - regulated in HCC tissues and cell lines （P 〈0. 05） . Over - ex-pression of ELMO1 promoted cell migration in SK - HEP - 1 cells, while knock - down of ELMO1 showed the opposite effect. Conclusion ELMO1 up - regulation significantly correlates with cell migration in hepatocellular carcinoma.

关 键 词：肝细胞癌 ELMO1 细胞迁移 

分 类 号：R-33[医药卫生] R735.7