HPLC-DAD-MS/MS检测止咳平喘类中药制剂中非法添加的12种化学药品  

Screening for 12 mixed illegally cough-relieving chemical composition in anti-cough and antiasthmatic traditional Chinese medicines by HPLC-DAD-MS/MS

作　　者：李奕婧 李晓琳[2] 韩家忠 徐玉文 

机构地区：[1]滨州市食品药品检验检测中心,山东滨州256602 [2]烟台毓璜顶医院,山东烟台264000 [3]山东省食品药品检验研究院,山东济南250101

出　　处：《药学学报》2017年第6期959-963,共5页Acta Pharmaceutica Sinica

基　　金：国家重大科学仪器设备开发专项资助项目(2012YQ180132)

摘　　要：建立止咳平喘类中药制剂中非法添加的12种化学药品的快速筛选方法。采用液相色谱-串联质谱法,以Kromasil100-5C18(250 mm×4.6 mm,5μm)为分析柱,流动相采用0.3%甲酸铵(用甲酸调至p H 2.97)和甲醇进行梯度洗脱,电喷雾电离(ESI)、全扫描一级质谱和全扫描二级质谱扫描方式,对止咳平喘类中药制剂中非法添加的12种化学药品进行筛选分析。建立了快速分离、检测止咳平喘类中药制剂中非法添加的12种化学药品的方法,检测限低于50 ng。该方法专属性强、灵敏度高,适用于止咳平喘类中药制剂中非法添加化学药品的筛选及确证工作。This study was designed to establish a high performance liquid chromatography （diode array detector, DAD）-tandem mass chromatography （HPLC-DAD-MS/MS） method for the simultaneously screening 12 kinds of cough-relieving chemical drugs illegally added in anti-cough and antiasthmatic traditional Chinese medicines. This method involved liquid chromatography-tandem mass spectrometry. The separation was conducted by Kromasil100-5C18 （250 mm × 4.6 mm, 5 μm） and the mobile phase was consisted of 0.3% ammonium formate （pH 2.97） and methanol. After separated by HPLC, the suspected components were analyzed by MS/MS and DAD and ultra scan was used to identify these illegally added drugs. A fast and sensitive HPLC-DAD-MS/MS method for the simultaneously screening for illegal chemical compositions was established. The LOD of these substances were below 50 ng. The method was sufficiently selective and sensitive to detect illegal chemical compositions in anti-cough and antiasthmatic traditional Chinese medicines.

关 键 词：止咳平喘中药制剂 HPLC-DAD-MS/MS 非法添加物 

分 类 号：R917[医药卫生—药物分析学]