熊果酸对HepG2增殖和凋亡的影响及部分机制研究  

Effect of ursolic acid on proliferation and apoptosis of hepatic carcinoma HepG2 cell line and its partial mechanism

作　　者：雷达[1] 李永利[1] 祁军安[1] 

机构地区：[1]陕西省宝鸡市中心医院肝胆胰脾外科,陕西宝鸡721008

出　　处：《中国现代普通外科进展》2017年第4期259-263,共5页Chinese Journal of Current Advances in General Surgery

基　　金：陕西省科学技术研究发展计划(2011K12-67)

摘　　要：目的:观察熊果酸对人肝癌细胞株HepG2增殖和凋亡的影响及其部分机制的研究。方法:将不同浓度熊果酸体外培养人肝癌HepG2细胞,通过MTT法检测熊果酸对细胞增殖抑制的情况,利用流式细胞检测术观察熊果酸对细胞凋亡及细胞周期的影响。同时利用Western blot法检测不同浓度熊果酸干预后人肝癌HepG2细胞pERK1/2蛋白、Cyclin D1蛋白的表达情况。结果:不同浓度熊果酸对人肝癌HepG2细胞的增殖均有抑制效应,并呈剂量、时间依赖性(P<0.05);60μmol/L熊果酸作用于人肝癌细胞株HepG2 72 h后达到最大细胞凋亡率(78.723±3.623)%。熊果酸可明显增加G_0/G_1期的细胞含量,诱导人肝癌细胞株HepG2的凋亡。熊果酸可抑制pERK1/2蛋白、Cyclin D1蛋白的表达,并随着浓度、时间逐渐上调抑制作用更加明显。结论:熊果酸可抑制人肝癌细胞株HepG2增殖,阻滞细胞期于G0/G1期,促进癌细胞凋亡,这一过程可能与下调细胞pERK1/2蛋白、Cyclin D1蛋白的表达有关。Objective: To investigate the effects of ursolic acid on proliferation and apoptosis of hepatic carcinoma cell line HepG2 and its partial mechanism. Methods: The human HepG2 cells were cultured by different concentrations of ursolic acid. The inhibitions of ursolic acid on cell proliferation were determined by using MTT. The effects of ursolic acid on on cell apoptosis and cell cyclewere detected by using flow cytometry. The expressions of pERK1/2 and Cyclin D1 proteins after culturing by different concentrations of ursolic acid were tested by using Western blotting. Results:The human hepatoma HepG2 cell proliferations were inhibited by different concentrations of ursolic acid, and the effects showed dose and time-dependent manner(P <0.05).The cell apoptosis rate for human hepatoma cell line HepG2 was achieved to the maximum under 60 μmol/L ursolic acid for 72 h, which was(78.723±3.623)%. Ursolic acid could significantly increase the G_0/G_1 phase cells proportion,and induce HepG2 apoptosis. Ursolic acid could inhibit the expressions of pERK1/2 and Cyclin D1 proteins, and inhibitory effects showed more apparent along with the concentration and time gradually increasing. Conclusion: Ursolic acid could inhibit the proliferation of human hepatic carcinoma HepG2 cell line, block of cells in G_0/G_1 phase, and promote the cell apoptosis. This might be related with down-regulation of the expressions of pERK1/2 and Cyclin D1 proteins.

关 键 词：熊果酸·HepG2细胞株·增殖·凋亡·pERKl/2蛋白·Cyciin D1蛋白 

分 类 号：R735.7[医药卫生—肿瘤]