转染CXCR7重组质粒对膀胱癌5637细胞株增殖和侵袭的影响  

Effect of CXCR7 recombinant plasmid on the proliferation and invasion of human bladder cancer cell line 5637

作　　者：曹倪豪[1] 周飞[1] 陆伟[1] 刘宁[2] 杨菲[1] Cao Nihao Zhou Fei Lu Wei et al(Department of Urology, Department of Endocrinology, Haimen People＇s Hospital of Jiangsu, Haimen 226100, China)

机构地区：[1]海门市人民医院泌尿外科、内分泌科,江苏海门226100 [2]东南大学附属中大医院泌尿外科,江苏南京210009

出　　处：《国际泌尿系统杂志》2017年第3期335-337,共3页International Journal of Urology and Nephrology

摘　　要：目的 探讨转染趋化因子受体CXCR7对膀胱癌细胞株5637增殖和凋亡的影响.方法 构建CXCR7表达质粒转染膀胱癌5637细胞,并通过四甲基偶氮唑蓝盐比色法（MTT）实验和Transwell小室实验与未转染组进行比较.结果 成功构建了CXCR7慢病毒表达载体.与空质粒组相比,转染CXCR7重组质粒能促进膀胱癌5637细胞中CXCR7的表达,通过MTT表明转染重组质粒组加强细胞的增殖能力和Transwell法检测表明穿过的细胞数显著增加,高表达CXCR7能够促进5637细胞的迁移能力.结论 CXCR7的高表达能提高膀胱癌细胞的增殖和侵袭能力,提示CXCR7与膀胱癌的发生、发展有关.Objectives To investigate the effect of CXCR7 on the growth and metastasis in 5637 cells.Methods Effective sequence of CXCR7 and the CXCR7shRNA were screened and constructed,and which transfected into 5637 cells.The adhesive ability was evaluated by MTT assay.The invasive ability was measured by Transwell experiment.Results High expression of CXCR7 was conducive to adhesive and invasive （P 〈 0.05）.Conclusions CXCR7 may be involved in bladder carcinoma carcinogenesis.

关 键 词：膀胱肿瘤 质粒 细胞增殖 

分 类 号：R737.14[医药卫生—肿瘤]