影响人颞下颌关节滑液间充质干细胞数量的相关因素分析  

Analysis of factors related to the number of mesenchymal stem cells derived from synovial fluid ofthe temporomandibular joint

作　　者：孙养鹏 郑有华[1] 张志光[1] Sun Yangpeng Zheng Youhua Zhang Zhiguang(Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University ＆ Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China)

机构地区：[1]中山大学光华口腔医学院·附属口腔医院口腔颌面外科·广东省口腔医学重点实验室,广州501155

出　　处：《中华口腔医学杂志》2017年第6期355-359,共5页Chinese Journal of Stomatology

摘　　要：目的初步筛选、分析影响人颞下颌关节滑液间充质干细胞数量的相关因素，以期为运用滑液间充质干细胞治疗颞下颌关节疾病奠定理论基础。方法收集2013年3月至2013年12月在中山大学光华口腔医学院颞下颌关节诊疗中心就诊的颞下颌关节紊乱病患者91例（共122个滑液标本）、就读中山大学北校区的全身健康志愿者6名（共6个滑液标本）及其相关临床信息，对滑液标本进行原代克隆形成并计数，应用Mann—WhitneyU秩和检验、Spearman相关分析统计分析滑液间充质干细胞的数量与供体年龄、关节健康状态、关节盘穿孔、髁突骨质破坏、滑液标本含血量以及疼痛视觉模拟评分值的关系。结果颞下颌关节滑液间充质干细胞的数量与疼痛视觉模拟评分值（r=0．041，P=0．672）、标本含血量（P=O．063）、髁突骨质破坏（P=O．371）均无显著相关；与年龄存在十分微弱的线性正相关关系（r=0．186，P=O．043）；在疾病状态下，颞下颌关节滑液中间充质干细胞数量显著上调（p=0．001）；关节盘穿孔组滑液间充质干细胞的数量显著高于非穿孔组（P=O．042）。结论滑液间充质干细胞的数量与关节内软组织结构的破坏密切相关。Objective To analyze related factors on the number of mesenchymal stem cells in the synovial fluid of the temporomandibular joint （TMJ） and provide an research basis for understanding of the source and biological role of mesenchymal stem cells derived from synovial fluid in TMJ. Methods One hundred and twenty-two synovial fluid samples from 91 temporomandibular disorders （TMD） patients who visited in Department of TMJ Center, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University from March 2013 to December 2013 were collected in this study, and 6 TMJ synovial fluid samples from 6 normal volunteers who were studying in the North Campus of Sun Yat-sen University were also collected, so did their clinical information. Then the relation between the number of mesenchymal stem cells derived from synovial fluid and the health status of the joints, age of donor, disc perforation, condylar bony destruction, blood containing and visual analogue scale score of pain were investigated using Mann-Whitney U test and Spearman rank correlation test. Results The number of mesenchymal stem cells derived from synovial fluid had no significant relation with visual analogue scale score of pain （r=O.041, P=-0.672）, blood containing （P=0.063）, condylar bony destruction （P= 0.371）. Linear correlation between the number of mesenchymal stem cells derived from synovial fluid and age of donor was very week （r=0.186, P=0.043）. The number of mesenchymal stem cells up-regulated when the joint was in a disease state （P=0.001）. The disc perforation group had more mesenchymal stem cells in synovial fluid than without disc perforation group （P=-0.042）. Conclusions The number of mesenchymal stem cells derived from synovial fluid in TMJ has no correlation with peripheral blood circulation and condylar bony destruction, while has close relation with soft tissue structure damage of the joint.

关 键 词：颞下颌关节 间质干细胞 滑液 滑膜 

分 类 号：R782.6[医药卫生—口腔医学]