转化生长因子β3联合牙髓干细胞在种植体骨结合中作用的实验研究  

Experimental study of transforming growth factor-β3 combined with dental pulp stem cells inpromoting the implant＇s osseointegration

作　　者：王腾[1] 木合塔尔·霍加[1] 李军[2] Wang Teng Muhetaer Huofia Li Jun(Department of Stomatology, People＇s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China （Wang T, Houjia M Department of Maxillofacial Surgery, People＇s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Chin)

机构地区：[1]新疆维吾尔自治区人民医院口腔科,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院颌面外科,乌鲁木齐830001

出　　处：《中华口腔医学杂志》2017年第6期367-373,共7页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81560180）

摘　　要：目的初步探讨转化生长因子β3 （transforming growth factor-β3, TGF-β3）联合体外培养的兔牙髓干细胞（dental pulp stem cells，DPSC）对增强种植体骨结合的影响，为缩短种植周期及增强骨结合提供新的视角。 方法将36只新西兰大白兔按随机数字表法随机分为磷酸盐缓冲液（phosphate buffer saline，PBS）组、DPSC组及TGF-β3＋DPSC组，每组12只。在兔下颌按随机数字表法随机拔除2颗牙齿，即刻植入种植体，PBS组植入Bio-Oss骨粉0.30 g＋PBS 20 μl; DPSC组植入Bio-Oss骨粉0.30 g＋ 1×108个/L DPSC 20 μl；TGF-β3＋DPSC组植入Bio-Oss骨粉0.30 g＋80 μg/L TGF-β3 20 μl＋1×108个/L DPSC 20 μl。术后4、8周分别处死新西兰大白兔18只，行茜素红染色检测成骨质量，行图像灰度分析检测骨涎蛋白、Ⅰ型胶原蛋白及骨钙蛋白的表达，行骨形态计量学分析检测种植体周围骨板宽度（combined bone lamella width，CBLW）、骨结合率（implant bone contact rate，IBCR）、骨小梁宽度（trabecular width, TW）及骨小梁视野面积百分比（trabecular area, TA）。 结果茜素红染色显示，术后4、8周，TGF-β3＋DPSC组较其他两组出现更多的红色钙化结节；术后8周较术后4周钙化结节进一步增多。图像灰度分析显示：术后4周，TGF-β3＋DPSC组骨涎蛋白、骨钙蛋白及Ⅰ型胶原相对表达量分别为（0.35±0.04）、（0.36±0.03）及（0.39±0.01）；DPSC组骨涎蛋白、骨钙蛋白及Ⅰ型胶原相对表达量分别为（0.27±0.02）、（0.24±0.01）及（0.28±0.03）；PBS组骨涎蛋白、骨钙蛋白及Ⅰ型胶原相对表达量分别为（0.13±0.03）、（0.15±0.02）及（0.16±0.02）。术后8周，TGF-β3＋DPSC组骨涎蛋白、骨钙蛋白及Ⅰ型胶原相对表达量分别为（0.51±0.02）、（0.49±0.03）及（0.53±0.02）；DPSC组骨涎蛋白、骨钙蛋白及Ⅰ型胶原相对表达量分别为（0.35±0.02）、（0.37±0.01）及（0.38±0.01）；PBS�Objective To investigate the effect of transforming growth factor-β3 （TGF-β3） and dental pulp stem cells （DPSC） in promoting the implant＇s osteointegration. Methods Thirty-three New Zealand white rabbits were randomly divided into phosphate buffer saline （PBS） group, DPSC group and TGF-β3 ±DPSC group （12 rabbits/group）. Two teeth from the rabbits＇s mandibular incisors or molars werepulled out randomly, then implant were placed in the tooth extraction site immediately, in PBS group, the implant area was filled with Bio-Oss powder 0.30 g mixed by PBS 20 Ixl only; while the implant area was filled with Bio-oss powder 0.30 g and lxl0~/L DPSC 20 txl in DPSC group; in the the TGF-I33±DPSC group the implant area was filled with Bio-Oss powder 0.30 g mixed with 1 x 108/L DPSC 20 pl and 80 pg/L TGF-133 20 p,1. Eighteen New Zealand rabbits were executed in the 4 weeks and 8 weeks respectively. The treated alveolar bone tissue and implant were collected for plastic section. Alizarin red staining （ARS）, immunohistochemical detection （IHC） Of bone sialoprotein （BSP）, osteocalcin （OC） and type I collagen （COL- I ） were performed after 4 weeks and 8 weeks. Combined bone lameha width （CBLW） and implant bone contact rate （IBCR）, trabecular width （TW） and trabecular area percentage （TA） were observed by histomorphometric measurement. Results ARS staining： 4 weeks after the operation, the TGF-[33 ±DPSC group showed more red calcified nodules than the other two groups; 8 weeks after operation, the red calcified nodule was further increased. 4 weeks after the operation, the expression of BSP, OC and COL- l was （0.35± 0.04）, （0.36 ± 0.03） and （0.39 ± 0.01） respectively in TGF-[33 ± DPSC group, （0.27 ± 0.02）, （0.24 ± 0.01） and （0.28±0.03） respectively in DPSC group, and （0.13±0.03）, （0.15±0.02） and （0.16±0.02） respectively in PBS group. Eight weeks ＇after operation, the expression of BSP, OC and COL- I was （0.51±0.02）, �

关 键 词：转化生长因子Β3 种植体 骨结合 牙髓干细胞 

分 类 号：R783.6[医药卫生—口腔医学]