AKT抑制剂MK-2206对卵巢癌SKOV3细胞增殖凋亡的影响  

Effects of AKT inhibitor MK-2206 on proliferation and apoptosis of ovarian cancer SKOV3 cells

作　　者：郭红艳[1] 张微[1] 黄玉琴[1] 习红丽[1] 

机构地区：[1]湖北医药学院附属襄阳医院妇产科,湖北襄阳441000

出　　处：《临床和实验医学杂志》2017年第12期1181-1184,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨MK-2206对卵巢癌SKOV3细胞增殖凋亡的影响及其作用机制。方法 MTT法检测MK-2206和顺铂单药及联合用药对SKOV3细胞增殖的影响,并计算半抑制浓度(IC50);FCM法检测MK-2206和顺铂单药及联合用药对SKOV3细胞凋亡的影响;Western-Blot法检测各组丝苏氨酸蛋白激酶(AKT)、雷帕霉素靶体蛋白(m TOR)及核糖体S6蛋白激酶(70S6K)蛋白的表达情况。结果 MK-2206和顺铂两单药均可抑制SKOV3的增殖,各浓度组具有统计学差异(P<0.05),联合用药时可显著降低顺铂半抑制浓度(P<0.05);同时两单药组可促进SKOV3细胞凋亡(P<0.05),联合用药时凋亡作用更显著(P<0.05);MK-2206和顺铂对SKOV3细胞中AKT、m-TOR及70S6K表达无明显影响,但可抑制p-AKT、p-m TOR及p-70S6K表达水平(P<0.05)。结论 MK-2206可抑制SKOV3细胞的增殖,促进其凋亡,并可增强SKOV3细胞对顺铂敏感性,其可能与抑制AKT、m TOR及70S6K磷酸化水平有关。Objective To investigate the effect of MK - 2206 on the proliferation and apoptosis and its mechanism of SK0V3 cells. Methods The proliferation inhibitory rates of SK0V3 cells treated with MK - 2206 or DDP alone and the combination of MK - 2206 and DDP were detected by MTT assay, and calculate the half inhibitory concentration （IC50）. The apoptosis rate of SKV3 cells treated with MK - 2206 or DDP alone and the combination of MK - 2206 and DDP were determined by flow cytometry. The expression levels of AKT, m - TOR and 70S6K proteins in SKOV3 cells were examined by Western - Blotting. Results MK - 2206 and cisplatin alone could inhibit the proliferation of SKOV3 , each concentration group has significant difference （ P 〈0 .05 ） , United could significantly reduce the half inhibitory concentration of cisplatin （ P 〈 0. 05）. MK - 2206 or DDP alone could promote apoptosis of SKOV3 cell （ P 〈 0.05） , MK - 2206 and cisplatin had no effect on AKT, mTOR and 70S6K expression in SKOV3 cells, but inhibited the expression of p - AKT, p - mTOR and p - 70S6K in SKOV3 cells （ P 〈 0 .05 ） . Con-clusion MK - 2206 inhibits SKOV3 cell proliferation and induce apoptosis, and may enhance the sensitivity of SKOV3 cells to cisplatin, which may be related to inhibition of AKT, mTOR and 70S6K phosphorylation levels.

关 键 词：卵巢癌 MK-2206 顺铂 SKOV3 

分 类 号：R737.31[医药卫生—肿瘤]